A functional and phenotypic comparison of murine natural killer (NK) cells and lymphokine‐activated killer (LAK) cells
A functional and phenotypic comparison of murine natural killer (NK) cells and lymphokine‐activated killer (LAK) cells
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小鼠自然杀伤 (NK) 细胞和淋巴因子激活杀伤 (LAK) 细胞的功能和表型比较
DOI：
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发表时间：
1989
影响因子：
6.4
通讯作者：
J. Roder
中科院分区：
文献类型：
--
作者：
D. Hoskin;J. Stankova;S. Anderson;J. Roder
淋巴因子激活的杀伤（LAK）细胞已被定义为白细胞介素2（IL-2）激活的细胞溶解效应细胞，对广泛的NK敏感性和NK抗性肿瘤细胞表现出非MHC限制性杀伤作用。关于LAK细胞是来源于NK细胞还是来源于一个独特的前体细胞群体，一直存在相当大的争论。在本研究中，我们比较了来源于T细胞去除的尼龙毛非贴壁脾细胞的LAK细胞与内源性NK细胞和用干扰素诱导剂polyl.C.激活的NK细胞，就其表型和功能特征而言。发现小鼠中主要的脾LAK前体是尼龙-羊毛-非粘附性、thyl-、MICG、-、Jlld.2、去唾液酸GM 1+细胞。这种表型由内源性NK细胞共享。大量活化的NK细胞除了表达去唾液酸GM 1外，还表达大分子不溶性冷球蛋白（MICG）。内源性和活化NK细胞均不表达在骨髓细胞、未成熟T细胞和大多数B细胞上发现的热稳定抗原，并由单克隆抗体（MA B）Jlld定义。2.然而，Jlld.2在一些LAK前体细胞和效应细胞上表达。去唾液酸GM 1标记物是所有LAK效应细胞共有的，而许多也是thyl+和/或MICG+。因此，LAK效应细胞是与NK细胞共有一些表型特征的异质群体。此外，在“高NK”和“低NK”小鼠品系中，LAK和NK活性之间存在正相关，表明NK细胞和LAK细胞具有共同的谱系。针对LFA-1 α和β链的单克隆抗体抑制LAK和活化的NK功能，而内源性NK和CTL杀伤仅受抗α链抗体的影响。与MHC限制性和非限制性CTL克隆一样，LAK细胞表达C11丝氨酸蛋白酶基因的mRNA转录物。我们的结论是，LAK细胞有几个共同的特点与NK细胞系，因此可能代表NK细胞在一个独特的激活状态。LAK细胞似乎采用与其他裂解细胞类型相同的细胞溶解机制。
Lymphokine‐activated killer (LAK) cells have been defined as interleukin 2 (lL‐2)‐activated cytolytic effector cells exhibiting non‐MHC restricted killing against a wide range of NK‐sensitive and NK‐resistant tumor cells. There has been considerable debate as to whether LAK cells are derived from NK cells or from a unique precursor population. In the present study, we compare LAK cells derived from T‐cell‐depleted nylon‐wool‐non‐adherent spleen cells with endogenous NK cells and NK cells activated with the interferon inducer polyl.C., in terms of their phenotype and functional characteristics. The predominant splenic LAK precursor in the mouse was found to be a nylon‐wool‐non‐adherent, thyl‐, MICG,‐,Jlld.2, asialo GM1+ cell. This phenotype is shared by endogenous NK cells. A significant number of activated NK cells express macromolecular insoluble cold globulin (MICG) in addition to asialo GM1. Neither endogenous nor activated NK cells express a heat‐stable antigen found on bone‐marrow cells, immature T cells, and most B cells and defined by monoclonal antibody (MAb)Jlld.2. However, Jlld.2 is expressed on some LAK precursor and effector cells. The asialo GM1 marker is common to all LAK effector cells, while many are also thyl+, and or MICG+. LAK effector cells are therefore a heterogeneous population sharing some phenotypic characteristics in common with NK cells. In addition, there is a positive correlation between LAK and NK activity in “high NK” and “low NK” mouse strains, suggesting that NK cells and LAK cells share a common lineage. Monoclonal antibodies to the alpha and beta chains of LFA‐1 inhibit LAK and activated NK function, while endogenous NK and CTL killing is affected only by anti‐alpha chain antibodies. LAK cells, like MHC‐restricted and non‐restricted CTL clones, express mRNA transcripts of the C11 serine protease gene. We conclude that LAK cells share several features in common with cells of the NK lineage and may therefore represent NK cells in a unique state of activation. LAK cells appear to employ cytolytic machinery common to other lytic cell types.
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鼠自然杀伤细胞和淋巴因子激活杀伤细胞之间的相似之处和区别。
影响因子：
4.3
作者：
Merluzzi,VJ;Smith,MD;Last-Barney,K
通讯作者：
Last-Barney,K
编码 T 细胞激活蛋白 TAP 的基因定位到 Ly-6 基因座。
DOI：
10.1073/pnas.83.9.2954
发表时间：
1986
影响因子：
11.1
作者：
Reiser,H;Yeh,ET;Gramm,CF;Benacerraf,B;Rock,KL
通讯作者：
Rock,KL
体内的纯化和靶细胞范围引发了母细胞自然杀伤细胞。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Biron,CA;Pedersen,KF;Welsh,RM
通讯作者：
Welsh,RM
一种可区分 T 细胞和 B 细胞亚群的单克隆抗体。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bruce,J;Symington,FW;McKearn,TJ;Sprent,J
通讯作者：
Sprent,J
LFA-1 抗原在人类 NK 克隆与靶细胞相互作用中的功能表征。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schmidt,RE;Bartley,G;Levine,H;Schlossman,SF;Ritz,J
通讯作者：
Ritz,J