The Cloning and Characterization of a New Stress Response Protein
The Cloning and Characterization of a New Stress Response Protein
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新应激反应蛋白的克隆和表征
DOI：
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发表时间：
1999
影响因子：
4.8
通讯作者：
M. Story
中科院分区：
文献类型：
--
作者：
R. Kodym;P. Calkins;M. Story
利用差异显示，在一种小鼠淋巴瘤细胞系中发现了一段过表达的cDNA片段，该细胞系在暴露于各种用于癌症治疗的药物后获得了对细胞死亡的抵抗力。克隆的1.1kb全长cDNA含有一个开放阅读框，编码一个以前未知的28 kDa哺乳动物蛋白p28。P28与一个包含谷胱甘肽S转移酶结构域的应激反应蛋白大家族有显著的同源性。与序列同源性相一致的是，p28与谷胱甘肽结合，但不能证明GST或谷胱甘肽过氧化物酶活性。Northern分析表明该蛋白在小鼠心脏和肝脏组织中大量表达，而抗p28抗体结合后在小鼠3T3细胞和早期传代的小鼠胚胎成纤维细胞中有p28的表达。亚细胞蛋白质组分显示p28定位于细胞质，但随着热应激的增加，p28重新定位到细胞蛋白质的核部分。根据序列同源性和蛋白质活性，我们认为p28作为一个小的应激反应蛋白，可能参与细胞氧化还原动态平衡，属于与θ类GSTs相关的GST样蛋白家族。
Using differential display, a cDNA fragment was identified as being overexpressed in a mouse lymphoma cell line that had gained resistance to cell death after exposure to a variety of agents used in cancer therapy. The full-length cDNA of 1.1 kb that was cloned contained an open reading frame coding for a previously unidentified 28-kDa mammalian protein, p28. p28 showed significant homologies to a large family of stress response proteins that contain a glutathione S-transferase (GST) domain. In correspondence with the sequence homology, p28 was found to bind glutathione; however, GST or glutathione peroxidase activity could not be demonstrated. Northern analysis of the mRNA of this protein showed abundant expression in mouse heart and liver tissues, whereas anti-p28 antibody binding identified p28 expression in mouse 3T3 cells and early passage mouse embryo fibroblasts. Subcellular protein fractionation revealed p28 localization in the cytoplasm, but with thermal stress p28 relocated to the nuclear fraction of cellular proteins. Based on sequence homology and protein activity we conclude that p28 acts as a small stress response protein, likely involved in cellular redox homeostasis, and belongs to a family of GST-like proteins related to class θ GSTs.
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影响因子：
11.2
作者：
Richard B. Lock;L. Stribinskiene
通讯作者：
Richard B. Lock;L. Stribinskiene
影响因子：
2.6
作者：
Michael D. Story;David Voehringer;C. Malone;M. Hobbs;Ray Meyn
通讯作者：
Michael D. Story;David Voehringer;C. Malone;M. Hobbs;Ray Meyn
去极化与血清素配对对蛋白质合成的影响，血清素是海兔联想学习的类似物。
DOI：
10.1073/pnas.91.10.4150
发表时间：
1994
影响因子：
11.1
作者：
Noel,F;Koumenis,C;Nunez-Regueiro,M;Raju,U;Byrne,JH;Eskin,A
通讯作者：
Eskin,A
DOI：
10.1073/pnas.95.6.2956
发表时间：
1998-03-17
影响因子：
11.1
作者：
Voehringer, DW;McConkey, DJ;Meyn, RE
通讯作者：
Meyn, RE