Non-enzymatic depurination of nucleic acids: factors and mechanisms.
Non-enzymatic depurination of nucleic acids: factors and mechanisms.
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DOI：
10.1371/journal.pone.0115950
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liang X
中科院分区：
文献类型：
--
作者：
An R;Jia Y;Wan B;Zhang Y;Dong P;Li J;Liang X
排尿与核酸的损伤和修复密切相关，长期以来一直受到人们的广泛关注。在本研究中，使用30个核苷酸的不同序列、不同pH值的短DNA片段池，用高效液相色谱(HPLC)分析去嘌呤。动力学分析结果表明，寡聚脱氧核苷酸的非酶脱纯反应符合典型的一级动力学，其温度依赖性符合阿累尼乌斯定律。我们的结果还清楚地表明，速率常数的对数与pH值之间的线性关系在pH 2.5附近有一个显著的点。有趣的是，出乎意料的是，脱氢作用在很大程度上依赖于DNA序列。研究发现，聚(Da)的去嘌呤速度非常慢，而胸腺嘧啶丰富序列的去嘌呤速度比其他序列快。这些结果在一定程度上可以用核苷酸碱基的质子化来解释。此外，根据我们的数据得到了两个方程，用于预测不同条件下的脱氨速率。这些结果为酸性胃液和某些酸性细胞器的基因突变和核酸代谢提供了基础数据，也可能有助于纠正人们对去嘌呤的一些误解。
Depurination has attracted considerable attention since a long time for it is closely related to the damage and repair of nucleic acids. In the present study, depurination using a pool of 30-nt short DNA pieces with various sequences at diverse pH values was analyzed by High Performance Liquid Chromatography (HPLC). Kinetic analysis results showed that non-enzymatic depurination of oligodeoxynucleotides exhibited typical first-order kinetics, and its temperature dependence obeyed Arrhenius’ law very well. Our results also clearly showed that the linear relationship between the logarithms of rate constants and pH values had a salient point around pH 2.5. Interestingly and unexpectedly, depurination depended greatly on the DNA sequences. The depurination of poly (dA) was found to be extremely slow, and thymine rich sequences depurinated faster than other sequences. These results could be explained to some extent by the protonation of nucleotide bases. Moreover, two equations were obtained based on our data for predicting the rate of depurination under various conditions. These results provide basic data for gene mutagenesis and nucleic acids metabolism in acidic gastric juice and some acidic organelles, and may also help to rectify some misconceptions about depurination.
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影响因子：
15
作者：
Baik, MH;Friesner, RA;Lippard, SJ
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作者：
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通讯作者：
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