Diesel exhaust particle exposure in vitro alters monocyte differentiation and function.
Diesel exhaust particle exposure in vitro alters monocyte differentiation and function.
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DOI：
10.1371/journal.pone.0051107
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sabroe I
中科院分区：
文献类型：
--
作者：
Chaudhuri N;Jary H;Lea S;Khan N;Piddock KC;Dockrell DH;Donaldson K;Duffin R;Singh D;Parker LC;Sabroe I
柴油机尾气颗粒造成的空气污染与哮喘和慢性阻塞性肺病等呼吸系统疾病患者的死亡率升高和住院率增加有关。在活动性炎症期间，单核细胞被募集到气道，并且可以取代驻留的肺泡巨噬细胞。因此，我们研究了慢性暴露于低浓度柴油机废气颗粒物14天是否可以改变健康个体和慢性阻塞性肺疾病患者单核细胞的表型和功能。从健康个体和诊断为慢性阻塞性肺病的人的血液中纯化单核细胞。单核细胞衍生的巨噬细胞在存在或不存在柴油机废气颗粒的情况下产生，并且通过研究它们的寿命、响应于Toll样受体激动剂和热灭活细菌的细胞因子产生以及表面标志物的表达来研究它们的表型。单核细胞衍生的巨噬细胞长期暴露于浓度>10 µg/ml的柴油机废气颗粒14天，导致线粒体和溶酶体功能障碍，以及健康和慢性阻塞性肺疾病个体中细胞随时间的逐渐损失。长期暴露于低浓度的柴油机废气颗粒损害CXCL 8细胞因子对脂多糖的反应，热杀死E。大肠杆菌，这种表型与CD 14和CD 11b表达的减少有关。因此，慢性柴油机排气颗粒暴露可能会改变肺巨噬细胞的数量和功能，这些巨噬细胞与健康人和慢性阻塞性肺疾病患者肺中局部募集的单核细胞不同。
Air pollution by diesel exhaust particles is associated with elevated mortality and increased hospital admissions in individuals with respiratory diseases such as asthma and chronic obstructive pulmonary disease. During active inflammation monocytes are recruited to the airways and can replace resident alveolar macrophages. We therefore investigated whether chronic fourteen day exposure to low concentrations of diesel exhaust particles can alter the phenotype and function of monocytes from healthy individuals and those with chronic obstructive pulmonary disease. Monocytes were purified from the blood of healthy individuals and people with a diagnosis of chronic obstructive pulmonary disease. Monocyte-derived macrophages were generated in the presence or absence of diesel exhaust particles and their phenotypes studied through investigation of their lifespan, cytokine generation in response to Toll like receptor agonists and heat killed bacteria, and expression of surface markers. Chronic fourteen day exposure of monocyte-derived macrophages to concentrations of diesel exhaust particles >10 µg/ml caused mitochondrial and lysosomal dysfunction, and a gradual loss of cells over time both in healthy and chronic obstructive pulmonary disease individuals. Chronic exposure to lower concentrations of diesel exhaust particles impaired CXCL8 cytokine responses to lipopolysaccharide and heat killed E. coli, and this phenotype was associated with a reduction in CD14 and CD11b expression. Chronic diesel exhaust particle exposure may therefore alter both numbers and function of lung macrophages differentiating from locally recruited monocytes in the lungs of healthy people and patients with chronic obstructive pulmonary disease.
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DOI：
10.1165/ajrcmb.23.4.3959
发表时间：
2000-10-01
影响因子：
6.4
作者：
Rusznak, C;Mills, PR;Lozewicz, S
通讯作者：
Lozewicz, S
影响因子：
1.7
作者：
Saito, Y;Azuma, A;Sugawara, I
通讯作者：
Sugawara, I
影响因子：
4.4
作者：
Perera, PY;Mayadas, TN;Vogel, SN
通讯作者：
Vogel, SN
影响因子：
5.8
作者：
Ishii H;Hayashi S;Hogg JC;Fujii T;Goto Y;Sakamoto N;Mukae H;Vincent R;van Eeden SF
通讯作者：
van Eeden SF
DOI：
10.1164/rccm.200402-235oc
发表时间：
2004-10-15
影响因子：
24.7
作者：
Goto, Y;Ishii, H;van Eeden, SF
通讯作者：
van Eeden, SF