Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets.
Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets.
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DOI：
10.1371/journal.pone.0005370
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Hall KD
中科院分区：
文献类型：
--
作者：
Guo J;Jou W;Gavrilova O;Hall KD
去除顺从饮食后，饮食诱导的肥胖会持续下去吗？我们对这个问题进行了研究，给雄性C57BL/6小鼠提供两种不同的顺从饮食，然后切换到饮食，以确定肥胖是否可逆。将雄性C57BL/6小鼠随机分为5组：1)C组，继续饲喂饲料；2)HF组，饲喂60%高脂饲料；3)EN组，高脂饲料+液体饲料；4)HF-C组，7周后从高脂转为饲喂；5)EN-C组，7周后从高脂+Enure®转为饲喂。所有的食物摄入量都是随意的。7周后，两组小鼠体重均增加(EN、HF和C组分别为44.6±0.65、39.8±0.63和28.6±0.63g)，并导致血清胰岛素、血糖和瘦素浓度升高，血清甘油三酯水平降低。在HF和EN小鼠中，肥胖的发展是由于能量摄入增加和平均能量输出相对减少以及步行活动减少所致。改吃食物后，HF-C组和EN-C组的体重有所下降，但随后与C组相比保持了持续性肥胖状态(分别为34.8±1.2，34.1±1.2比30.8±0.8克；P<；0.05)，体脂增加了40%-50%。除了瘦素升高的趋势外，所有的血清激素和代谢物都恢复到对照水平。HF-C组和EN-C组的平均能量输出与C组一致，尽管研究结束时能量摄入量增加不到1千卡/天，但持续性肥胖仍保持不变。我们的结果说明了在确定体重时考虑能量不平衡的历史的重要性，以及在取消顺应性饮食后体重持续上升的原因可能是能量摄入量极小的增加。
Does diet-induced obesity persist after an obesigenic diet is removed? We investigated this question by providing male C57BL/6 mice with free access to two different obesigenic diets followed by a switch to chow to determine if obesity was reversible. Male C57BL/6 mice were randomly assigned to five weight-matched groups: 1) C group that continuously received a chow diet; 2) HF group on a 60% high fat diet; 3) EN group on the high fat diet plus liquid Ensure®; 4) HF-C group switched from high fat to chow after 7 weeks; 5) EN-C group switched from high fat plus Ensure® to chow after 7 weeks. All food intake was ad libitum. Body weight was increased after 7 weeks on both obesigenic diets (44.6±0.65, 39.8±0.63, and 28.6±0.63 g for EN, HF, and C groups, respectively) and resulted in elevated concentrations of serum insulin, glucose, and leptin and lower serum triglycerides. Development of obesity in HF and EN mice was caused by increased energy intake and a relative decrease of average energy output along with decreased ambulatory activity. After the switch to chow, the HF-C and EN-C groups lost weight but subsequently maintained a state of persistent obesity in comparison to the C group (34.8±1.2, 34.1±1.2 vs. 30.8±0.8 g respectively; P<0.05) with a 40–50% increase of body fat. All serum hormones and metabolites returned to control levels with the exception of a trend for increased leptin. The HF-C and EN-C groups had an average energy output in line with the C group and the persistent obesity was maintained despite a non-significant increase of energy intake of less than 1 kcal/d at the end of the study. Our results illustrate the importance of considering the history of energy imbalance in determining body weight and that a persistent elevation of body weight after removal of obesigenic diets can result from very small increases of energy intake.
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影响因子：
7.7
作者：
Biddinger, SB;Almind, K;Kahn, CR
通讯作者：
Kahn, CR
影响因子：
56.9
作者：
Hill, JO;Peters, JC
通讯作者：
Peters, JC
影响因子：
15.9
作者：
KESSLER, JI
通讯作者：
KESSLER, JI
影响因子：
4.8
作者：
Chen, M;Haluzik, M;Weinstein, LS
通讯作者：
Weinstein, LS
DOI：
10.1086/515876
发表时间：
1997-11-01
期刊：
PHYSIOLOGICAL ZOOLOGY
影响因子：
--
作者：
Corp, N;Gorman, ML;Speakman, JR
通讯作者：
Speakman, JR