ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox
ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox
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ROS诱导的氧化应激和类凋亡事件直接影响百子莲冻存胚性愈伤组织的细胞活力
DOI：
10.1007/s00299-015-1802-0
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发表时间：
2015-06
影响因子：
6.2
通讯作者：
Xiao-hui Shen
中科院分区：
文献类型：
--
作者：
Guan-qun Chen;Jie Zhang;Barbara M. Reed;Xiao-hui Shen
氧化胁迫和H2 O2诱导的类凋亡细胞程序性死亡是植物超低温保存过程中细胞损伤的两个关键因素。它们的抑制可以提高细胞活力。我们推测，在超低温保存过程中，由ROS诱导的氧化应激和类凋亡事件严重影响植物细胞的活力。研究百子莲胚性愈伤组织超低温保存过程中细胞形态和超微结构的变化，以及超低温保存过程中ROS（O2（·-）、H2 O2和OH·）、抗氧化系统和程序性细胞死亡（PCD）的动态变化。质膜，细胞器超微结构的变化，并在丙二醛（膜脂质过氧化产物）的增加表明，氧化损伤和PCD事件发生在几个早期的冷冻保存步骤。PCD事件包括自噬、类凋亡和坏死也发生在冷冻保存的后期阶段，并且大多数是凋亡。H2 O2是介导氧化损伤和影响细胞活力的最重要的活性氧分子，过氧化氢酶和AsA-GSH循环参与清除细胞内H2 O2，保护细胞免受应激损伤的全过程。基因表达研究证实了抗氧化系统和PCD相关基因在冻存过程的主要步骤中的变化，这些变化与提高细胞活力相关。降低氧化应激或通过使蛋白酶失活来抑制凋亡样事件将冻存细胞的存活率分别从49.14%提高到86.85%和89.91%。这些结果验证了我们的模型ROS诱导的氧化应激和类凋亡事件在植物超低温保存。本研究为深入了解低温保存过程中细胞的应激反应机制提供了新的思路。
Oxidative stress and apoptosis-like programmed cell death, induced in part by H 2 O 2 , are two key factors that damage cells during plant cryopreservation. Their inhibition can improve cell viability. We hypothesized that oxidative stress and apoptosis-like event induced by ROS seriously impact plant cell viability during cryopreservation. This study documented changes in cell morphology and ultrastructure, and detected dynamic changes in ROS components (O 2 (·-) , H2O2 and OH·), antioxidant systems, and programmed cell death (PCD) events during embryonic callus cryopreservation of Agapanthus praecox. Plasmolysis, organelle ultrastructure changes, and increases in malondialdehyde (a membrane lipid peroxidation product) suggested that oxidative damage and PCD events occurred at several early cryopreservation steps. PCD events including autophagy, apoptosis-like, and necrosis also occurred at later stages of cryopreservation, and most were apoptosis. H2O2 is the most important ROS molecule mediating oxidative damage and affecting cell viability, and catalase and AsA-GSH cycle are involved in scavenging the intracellular H2O2 and protecting the cells against stress damage in the whole process. Gene expression studies verified changes of antioxidant system and PCD-related genes at the main steps of the cryopreservation process that correlated with improved cell viability. Reducing oxidative stress or inhibition of apoptosis-like event by deactivating proteases improved cryopreserved cell viability from 49.14 to 86.85 % and 89.91 %, respectively. These results verify our model of ROS-induced oxidative stress and apoptosis-like event in plant cryopreservation. This study provided a novel insight into cell stress response mechanisms in cryopreservation.
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DOI：
10.1016/j.plantsci.2013.07.011
发表时间：
2013-11
期刊：
Plant science : an international journal of experimental plant biology
影响因子：
--
作者：
L. Ren;Di Zhang;Xiang-Ning Jiang;Y. Gai;Wei-ming Wang;B. Reed;Xiao-hui Shen
通讯作者：
L. Ren;Di Zhang;Xiang-Ning Jiang;Y. Gai;Wei-ming Wang;B. Reed;Xiao-hui Shen
影响因子：
6.2
作者：
E. Uchendu;S. Leonard;M. Traber;B. Reed
通讯作者：
E. Uchendu;S. Leonard;M. Traber;B. Reed
DOI：
10.1007/978-0-387-72276-4_3
发表时间：
2008
期刊：
--
影响因子：
--
作者：
A. Sakai;D. Hirai;T. Niino
通讯作者：
A. Sakai;D. Hirai;T. Niino
DOI：
--
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Yue Wang;Xianli Fan;Gao Wang;Di Zhang;Xiao-hui Shen
通讯作者：
Yue Wang;Xianli Fan;Gao Wang;Di Zhang;Xiao-hui Shen
影响因子：
4.3
作者：
M. Skyba;L. Petijová;J. Košuth;D. Koleva;T. Ganeva;V. Kapchina-Toteva;E. Čellárová
通讯作者：
M. Skyba;L. Petijová;J. Košuth;D. Koleva;T. Ganeva;V. Kapchina-Toteva;E. Čellárová