Pleiotropic effects of trans-regulatory mutations on fitness and gene expression.
Pleiotropic effects of trans-regulatory mutations on fitness and gene expression.
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DOI：
10.1126/science.abj7185
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发表时间：
2022-07
期刊：
影响因子：
56.9
通讯作者：
Wittkopp, Patricia J.
中科院分区：
文献类型：
--
作者：
Vande Zande, Petra;Hill, Mark S.;Wittkopp, Patricia J.
基因表达的变异来自顺式和反式调节突变，其差异性地导致表达差异。在这里，我们比较了在酿酒酵母中的顺式和反式调控突变对基因表达和适应性的影响，重点是TDH 3基因。我们使用顺式调节突变的影响来推断可归因于焦点基因以外的影响的反式调节突变的影响，揭示了多效性效应的分布。顺式和反式调节突变对基因表达有不同的影响，反式调节突变体的多效性影响了与焦点基因平行和下游的基因表达。我们观察到的反式调节突变的更广泛和有害的影响是一致的，随着进化时间的推移，它们对表达差异的相对贡献逐渐减少。这项研究量化了多效性的影响，反式调节突变的健身和基因表达相对于一个焦点基因。
Variation in gene expression arises from cis- and trans-regulatory mutations, which contribute differentially to expression divergence. Here, we compare the impacts on gene expression and fitness for cis- and trans-regulatory mutations in Saccharomyces cerevisiae, with a focus on the TDH3 gene. We use the effects of cis-regulatory mutations to infer effects of trans-regulatory mutations attributable to impacts beyond the focal gene, revealing a distribution of pleiotropic effects. Cis- and trans-regulatory mutations had different effects on gene expression, with pleiotropic effects of trans-regulatory mutants impacting expression of genes both in parallel to and downstream of the focal gene. The more widespread and deleterious effects of trans-regulatory mutations we observed are consistent with their decreasing relative contribution to expression differences over evolutionary time. This study quantifies the pleiotropic impacts of trans-regulatory mutations on fitness and gene expression relative to a focal gene.
影响因子：
7
作者：
Coolon JD;McManus CJ;Stevenson KR;Graveley BR;Wittkopp PJ
通讯作者：
Wittkopp PJ
影响因子：
7.7
作者：
Duveau F;Vande Zande P;Metzger BP;Diaz CJ;Walker EA;Tryban S;Siddiq MA;Yang B;Wittkopp PJ
通讯作者：
Wittkopp PJ
影响因子：
7.7
作者：
Duveau F;Hodgins-Davis A;Metzger BP;Yang B;Tryban S;Walker EA;Lybrook T;Wittkopp PJ
通讯作者：
Wittkopp PJ