The CentriMag centrifugal blood pump as a benchmark for in vitro testing of hemocompatibility in implantable ventricular assist devices.
The CentriMag centrifugal blood pump as a benchmark for in vitro testing of hemocompatibility in implantable ventricular assist devices.
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DOI：
10.1111/aor.12351
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发表时间：
2015-02
影响因子：
2.4
通讯作者：
Thornton CA
中科院分区：
文献类型：
--
作者：
Chan CH;Pieper IL;Hambly R;Radley G;Jones A;Friedmann Y;Hawkins KM;Westaby S;Foster G;Thornton CA
可植入的心室辅助装置(VAD)已被证明对晚期心力衰竭患者是有效的，作为通往移植或目的地治疗的桥梁。然而，VAD的使用往往会导致感染、出血和血栓形成，这些副作用可归因于对血细胞和血浆蛋白的损害。单靠测量溶血并不能提供足够的信息来了解血液的总损害，需要研究现有泵对更广泛的血细胞类型和血浆蛋白(如von Willebrand因子(VWF))的影响，以加深我们对更安全泵设计的理解。体外CentriMag(Thoratec Corporation，Pleasanton，CA，USA)在100毫米汞柱下的标准溶血水平低于0.01g/100 L，但对白细胞、vWF多聚体和血小板的影响尚不清楚。在这里，CentriMag用牛血(n=15)在恒定的血流动力学条件下进行了测试，并与静态对照的血细胞总数、溶血、白细胞死亡、vWF多聚体、微粒、血小板激活和细胞凋亡进行了比较。体外培养6h后，CentriMag可显著降低健康白细胞(P<；0.006)、诱导白细胞微粒(P<；10−5)和vWF多聚体大分子水平(P<；10−5)。尽管有白细胞损伤、微粒形成和vWF多聚体的切割，这些结果表明CentriMag是一种血液相容性泵，可以作为血液损伤检测的标准，为新型植入式血泵的设计提供信息。
Implantable ventricular assist devices (VADs) have proven efficient in advanced heart failure patients as a bridge-to-transplant or destination therapy. However, VAD usage often leads to infection, bleeding, and thrombosis, side effects attributable to the damage to blood cells and plasma proteins. Measuring hemolysis alone does not provide sufficient information to understand total blood damage, and research exploring the impact of currently available pumps on a wider range of blood cell types and plasma proteins such as von Willebrand factor (vWF) is required to further our understanding of safer pump design. The extracorporeal CentriMag (Thoratec Corporation, Pleasanton, CA, USA) has a hemolysis profile within published standards of normalized index of hemolysis levels of less than 0.01 g/100 L at 100 mm Hg but the effect on leukocytes, vWF multimers, and platelets is unknown. Here, the CentriMag was tested using bovine blood (n = 15) under constant hemodynamic conditions in comparison with a static control for total blood cell counts, hemolysis, leukocyte death, vWF multimers, microparticles, platelet activation, and apoptosis. The CentriMag decreased the levels of healthy leukocytes (P < 0.006), induced leukocyte microparticles (P < 10−5), and the level of high molecular weight of vWF multimers was significantly reduced in the CentriMag (P < 10−5) all compared with the static treatment after 6 h in vitro testing. Despite the leukocyte damage, microparticle formation, and cleavage of vWF multimers, these results show that the CentriMag is a hemocompatible pump which could be used as a standard in blood damage assays to inform the design of new implantable blood pumps.
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影响因子：
4.6
作者：
Crow, Sheri;Chen, Dong;Villamizar-Ortiz, Nestor
通讯作者：
Villamizar-Ortiz, Nestor
影响因子：
2.1
作者：
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通讯作者：
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影响因子：
9.7
作者：
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Strueber, Martin
影响因子：
8.9
作者：
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通讯作者：
Frazier, OH
影响因子：
2.4
作者：
Chan, Chris H. H.;Hilton, Andrew;Hawkins, Karl
通讯作者：
Hawkins, Karl