Oxidation of aromatic compounds and bioelectrocatalysis of peroxide by a novel white laccase from Myrothecium verrucaria NF-05

Oxidation of aromatic compounds and bioelectrocatalysis of peroxide by a novel white laccase from Myrothecium verrucaria NF-05
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来自疣状漆斑菌 NF-05 的新型白色漆酶对芳香族化合物的氧化和过氧化物的生物电催化

DOI:
10.1016/j.catcom.2012.11.010
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发表时间:
2013-01
影响因子:
3.7
通讯作者:
Min Zhao
Min Zhao
中科院分区:
化学3区
文献类型:
--
作者:
Dan Zhao;Dai-Zong Cui;Xi Zhang;Min Zhao

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研究了疣孢漆斑菌(Myrothecium verrucaria NF-05)产白色漆酶的酶学性质和电化学催化性能,并与商业氧化还原酶进行了比较。NF-05漆酶能有效地催化多种酚类和胺类化合物的氧化,具有广泛的底物特异性。循环伏安曲线表明,NF-05漆酶直接吸附在碳电极上,产生直接的电子转移。NF-05漆酶修饰电极上的过氧化氢生物电化学更有效地比用商业酶修饰的电极上。结果表明,该酶在芳香族污染物的环境生物修复和电子器件制备中具有潜在的应用前景。
The enzymatic and electrochemical catalytic properties of a novel white laccase from Myrothecium verrucaria NF-05 were evaluated and compared with those of commercial oxidoreductases. NF-05 laccase effectively catalyzed the oxidation of several phenols and amines, indicating its wide substrate specificity. The cyclic voltammetry curves showed direct electron transfer on carbonous electrodes due to the direct absorption of NF-05 laccase. Peroxide was bioelectrochemically reduced more efficiently on NF-05 laccase-modified electrodes than on electrodes that were modified with commercial enzymes. The results demonstrate the potential applications of this enzyme in environmental bioremediation of aromatic pollutants and preparation of electronic devices.
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