Versatile confocal/optical coherence tomography system for embryonic developmental imaging

Versatile confocal/optical coherence tomography system for embryonic developmental imaging
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用于胚胎发育成像的多功能共焦/光学相干断层扫描系统

DOI:
10.1117/12.765824
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发表时间:
2008
期刊:
--
影响因子:
--
通讯作者:
Bradu A
Bradu A
中科院分区:
--
文献类型:
--
作者:
Bradu A

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设计并搭建了一套光学相干层析成像系统,用于获取散射生物样品的图像。通过同时获取和显示高分辨率的正面(C-扫描)OCT和激光扫描共聚焦图像的果蝇胚胎,我们证明了该系统的潜力被用作一个强大的工具,在果蝇胚胎发育成像。该系统同样可以用于无创可视化和测量果蝇幼虫心脏的运动,并可以很容易地切换到OCT B扫描制度。共聚焦通道增加了引导,因为样本可以被快速定位,这使得该系统在大规模基因筛选中的使用是可行的。
An Optical Coherence Tomography (OCT) imaging system has been designed and constructed to acquire images of scattering biological samples. By simultaneously acquiring and displaying high resolution en-face (C-scan) OCT and Laser Scanning Confocal images of Drosophila melanogaster embryos we demonstrate the potential of the system to be used as a powerful tool for imaging in Drosophila embryonic development. The system can equally be used for non invasive visualizations and measurements of the movement of Drosophila melanogaster larval heart and can easily be switched in the OCT B-scan regime. The confocal channel adds guidance as the specimen can be quickly located and this makes the use of the system in a large scale gene screen feasible.
DOI: 10.1364/ol.23.000147
发表时间: 1998-02-01
期刊: OPTICS LETTERS
影响因子: 3.6
作者:
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通讯作者: Jackson, DA
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发表时间: 2002
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影响因子: --
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影响因子: 56.9
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