Fibrotic and inflammatory characteristics of epidural fat adjacent to the ossification area in patients with ossification of the ligament flavum.
Fibrotic and inflammatory characteristics of epidural fat adjacent to the ossification area in patients with ossification of the ligament flavum.
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黄韧带骨化患者骨化区附近硬膜外脂肪的纤维化和炎症特征。
DOI：
10.1002/jsp2.1229
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发表时间：
2022-12
期刊：
影响因子：
3.7
通讯作者：
Liu, Xiaoguang
中科院分区：
文献类型：
--
作者：
Dou, Xinyu;Mao, Tianli;Ma, Yunlong;Jia, Fei;Liu, Yu;Liu, Xiaoguang
观察胸椎黄韧带骨化(TOF)患者骨化区附近硬膜外脂肪(EF)的组织学和炎症特点，为探讨EF对OLF的影响提供初步的研究依据。Tolf患者(n=26)和非Tolf患者(n=23)的EF和自体皮下脂肪组织(SCAT)在胸椎后路手术中采集。组织学检测脂肪细胞大小和纤维化程度。免疫组织化学染色检测血管形成和炎性细胞浸润情况。实时荧光定量聚合酶链式反应分析成脂相关基因。采用酶联免疫吸附试验检测EF条件培养液中IL-6、肿瘤坏死因子-α和瘦素的分泌。用Western印迹法检测黄韧带(LF)中磷酸化STAT3蛋白的表达。Tolf组和非Tolf组EF的脂肪细胞大小相似，但显著小于自体SCAT组。EF组成脂相关基因的表达低于SCAT组。Tolf组较非Tolf组有更多的纤维化和血管形成。Tolf组EF可见较多巨噬细胞和B淋巴细胞浸润。TOF组EF分泌的细胞因子IL-6、肿瘤坏死因子-α和瘦素水平显著高于非TOF组。Tolf组LFSTAT3磷酸化水平显著上调。在形态上，邻近骨化区的EF比自体SCAT更小、更均匀，表现出与内脏脂肪的特征相似。Tolf组的EF表现出更多的纤维化、血管化和炎症性表型，分泌多种细胞因子。Tolf组磷酸化的STAT3蛋白表达显著上调。EF的这些性质是否直接影响OLF的过程还有待进一步探讨。观察胸椎黄韧带骨化(TOF)患者骨化区附近硬膜外脂肪(EF)的组织学和炎症特点，为探讨EF对OLF的影响提供初步依据。Tolf组和非Tolf组的EF在形态上没有显著差异，但两组的EF比自体SCAT更小、更均匀，类似于内脏脂肪组织(VAT)。此外，EF的遗传特征与VAT非常相似。Tolf组的EF纤维化和血管形成比非Tolf组更明显。此外，Tolf的EF具有更明显的特征，具有炎性表型，可分泌多种炎性介质。这些发现表明EF在OLF的发生中有潜在的作用。
To observe histological and inflammatory characteristics of epidural fat (EF) adjacent to the ossification area in patients with ossification of the thoracic ligament flavum (TOLF) and provide a preliminary research basis for investigating the impact of the EF on OLF. Samples of EF and autologous subcutaneous adipose tissue (SCAT) from TOLF patients (n = 26) and non‐TOLF patients (n = 23) were harvested during posterior thoracic spine surgery. Adipocyte size and fibrosis were measured by histology. Vascularization and inflammatory cell infiltration were evaluated by immunohistochemical staining. Adipogenesis‐related genes were assessed by real‐time quantitative PCR. Conditioned media from cultured EF were evaluated via enzyme‐linked immunosorbent assay to detect the secretion of inflammatory cytokines, including interleukin‐6 (IL‐6), tumor necrosis factor (TNF‐α), and leptin. The phosphorylated STAT3 protein level in ligament flavum (LF) was examined using Western blot. Adipocytes size in EF was similar between in the TOLF and non‐TOLF groups, but significantly smaller than that from autologous SCAT. Adipogenesis‐related mRNA expression in EF was lower than that in SCAT. More fibrosis and vascularization were found in TOLF than in non‐TOLF. EF in the TOLF group exhibited more macrophages and B lymphocytes infiltrated. The levels of cytokines such as IL‐6, TNF‐α, and leptin secreted by EF were significantly higher in the TOLF group than non‐TOLF. The level of phosphorylated STAT3 in LF was significantly upregulated in the TOLF group. Morphologically, EF adjacent to the ossification area is smaller and more uniform than autologous SCAT, exhibiting a characteristic similarity to visceral fat. EF in the TOLF group shows a more fibrotic, vascularized, and inflammatory phenotype, which secretes multiple cytokines. The phosphorylated STAT3 protein was significantly upregulated in the TOLF group. Whether these properties of EF directly affect the process of OLF needs to be further explored. We observed the histological and inflammatory characteristics of epidural fat (EF) adjacent to the ossification area in patients with ossification of the thoracic ligament flavum (TOLF) and provide a preliminary basis for investigating the impact of EF on OLF. Morphologically, EF exhibits no significant differences between the TOLF and non‐TOLF groups, but EF from both groups was smaller and more uniform than autologous SCAT, similar to visceral adipose tissue (VAT). Additionally, the genetic characteristics of EF were very similar to those of VAT. EF fibrosis and vascularization were more apparent in the TOLF group than in the non‐TOLF group. In addition, EF in TOLF showed more distinct characteristics, with an inflammatory phenotype, and could secrete many inflammatory mediators. These findings indicate that EF has a potential role in the occurrence of OLF.
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影响因子：
7.7
作者：
Garin-Shkolnik, Tali;Rudich, Assaf;Rubinstein, Menachem
通讯作者：
Rubinstein, Menachem
影响因子：
13.3
作者：
Eymard, Florent;Pigenet, Audrey;Houard, Xavier
通讯作者：
Houard, Xavier
DOI：
10.1073/pnas.0601752103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Gesta, S;Blüher, M;Kahn, CR
通讯作者：
Kahn, CR
影响因子：
4
作者：
Lin, Cheng-Yi;Liu, Tse-Ying;Chen, Ming-Hong
通讯作者：
Chen, Ming-Hong
影响因子：
56.9
作者：
Hong, JH;Hwang, ES;Yaffe, MB
通讯作者：
Yaffe, MB