Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.
Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.
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DOI：
10.1371/journal.pone.0126823
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Buhman KK
中科院分区：
文献类型：
--
作者：
D'Aquila T;Sirohi D;Grabowski JM;Hedrick VE;Paul LN;Greenberg AS;Kuhn RJ;Buhman KK
小肠对膳食脂肪的吸收是一个多步骤的过程，它调节必需营养素和能量的摄取和输送。该过程的一个步骤是将膳食脂肪暂时储存在细胞质脂滴（CLD）中。膳食脂肪的储存和动员被认为是由与CLD相关的蛋白质调节的;然而，该过程的机制细节目前尚不清楚。在这项研究中，我们分析了蛋白质组的CLDs分离肠细胞收获的小鼠小肠后，饮食脂肪的挑战。在这项分析中，我们鉴定了181种与CLD组分相关的蛋白质，其中37种与已知的脂质相关代谢途径相关。我们通过共聚焦和电子显微镜证实了这些蛋白质中的几种在CLD上或周围的定位，包括周脂蛋白3、载脂蛋白A-IV和酰基辅酶A合成酶长链家族成员5。肠上皮细胞CLD蛋白质组的鉴定为CLD代谢的潜在调节剂和膳食脂肪吸收过程提供了新的见解。
Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-CoA synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption.
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影响因子：
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