Case Report: Genomic Characteristics of the First Known Case of SARS-CoV-2 Imported From Spain to Sichuan, China.
Case Report: Genomic Characteristics of the First Known Case of SARS-CoV-2 Imported From Spain to Sichuan, China.
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病例报告：从西班牙输入到中国四川的首例已知 SARS-CoV-2 病例的基因组特征
DOI：
10.3389/fmed.2021.783646
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发表时间：
2021
影响因子：
3.9
通讯作者：
Jia X
中科院分区：
文献类型：
--
作者：
Liu C;Tang B;Gao C;Deng J;Shen M;Li C;Fu Z;Gao Z;Jiang Q;Shi H;He M;Jiang H;Jia X
2020年3月以来，由严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）引起的大流行在中国已基本得到控制，但国产SARS-CoV-2的输入开始增加。该研究报告了2020年3月11日从西班牙输入中国四川省的首例SARS-CoV-2无症状感染病例。这名受感染的男性体温为37.5 ℃，血氧饱和度正常，计算机断层扫描（CT）检查显示他的肺部没有阴影。然而，使用qPCR检测，受试者的咽拭子检测结果为SARS-CoV-2阳性。在本研究中，我们对受试者的呼吸道咽拭子进行了转录组测序，发现SARS-CoV-2的优势序列（Gene Bank ID：MW 301121）是目前全球流行的刺突蛋白D 614 G突变株。我们下载并分析了从中国和西班牙病例中收集的SARS-CoV-2序列，以进行比较和追踪。2020年3月11日以后，中国国内进化枝自然分为输入的SARS-CoV-2 D 614 G突变株和进化相关的相似序列与原武汉地区收集的序列。本研究报道的序列位于一个小分支上，与武汉序列的进化距离较远。正如预期的那样，鉴定的序列与在西班牙流行的SARS-CoV-2 D 614 G突变株的进化密切相关。
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been basically under control in China since March 2020, but the import of domestic SARS-CoV-2 has begun to increase. This study reported the first case of asymptomatic SARS-CoV-2 infection imported from Spain into Sichuan Province, China, on March 11, 2020. The infected male had a body temperature of 37.5°C, normal blood oxygen saturation levels, and a computed tomography (CT) examination showed that his lungs had no shadows. However, a throat swab from the subject tested positive for SARS-CoV-2 using qPCR assay. In this study, we conducted transcriptome sequencing on respiratory throat swabs from the subject and found that the dominant SARS-CoV-2 sequence (Gene Bank ID: MW301121) was a spike protein D614G mutant strain, which is currently popular throughout world. We downloaded and analyzed SARS-CoV-2 sequences collected from cases in China and Spain for comparison and tracing purposes. After March 11, 2020, the Chinese domestic clade was naturally divided into the imported SARS-CoV-2 D614G mutant strain and evolutionarily-related similar sequences and that of sequences collected in the original Wuhan area. The sequence reported in this study was located on a small branch, far from the evolution of Wuhan sequences. As expected, the identified sequence was closely related to the evolution of the SARS-CoV-2 D614G mutant strain circulating in Spain.
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影响因子：
4.9
作者：
Gómez-Carballa A;Bello X;Pardo-Seco J;Pérez Del Molino ML;Martinón-Torres F;Salas A
通讯作者：
Salas A
影响因子：
4.6
作者：
Isabel, Sandra;Grana-Miraglia, Lucia;Poutanen, Susan M.
通讯作者：
Poutanen, Susan M.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
48
作者：
Kim D;Langmead B;Salzberg SL
通讯作者：
Salzberg SL
影响因子：
7
作者：
Gómez-Carballa A;Bello X;Pardo-Seco J;Martinón-Torres F;Salas A
通讯作者：
Salas A