Binary Classifier for Computing Posterior Error Probabilities in MetaMorpheus.
Binary Classifier for Computing Posterior Error Probabilities in MetaMorpheus.
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DOI：
10.1021/acs.jproteome.0c00838
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发表时间：
2021-04-02
影响因子：
4.4
通讯作者：
Smith LM
中科院分区：
文献类型：
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作者：
Shortreed MR;Millikin RJ;Liu L;Rolfs Z;Miller RM;Schaffer LV;Frey BL;Smith LM
MetaMorpheus is a free, open-source software program for the identification of peptides and proteoforms from data-dependent acquisition, tandem MS experiments. There is inherent uncertainty in these assignments for several reasons including: limited overlap between experimental and theoretical peaks; m/z uncertainty; and noise peaks or peaks from co-isolated peptides that produce false matches. False discovery rates provide only a set-wise approximation for incorrect spectrum matches. Here, we implemented a binary decision tree calculation within MetaMorpheus to compute a posterior error probability, which provides a measure of uncertainty for each peptide spectrum match. We demonstrate its utility for increasing identifications and resolving ambiguities in bottom-up, top-down, proteogenomic and non-specific digestion searches.
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影响因子：
4.4
作者：
Granholm, Viktor;Noble, William Stafford;Kall, Lukas
通讯作者：
Kall, Lukas
影响因子：
3
作者：
Granholm V;Noble WS;Käll L
通讯作者：
Käll L
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
4.4
作者：
Li Q;Shortreed MR;Wenger CD;Frey BL;Schaffer LV;Scalf M;Smith LM
通讯作者：
Smith LM