Producer cell-dependent requirement of the Nef protein for efficient entry of HIV-1 into cells.
Producer cell-dependent requirement of the Nef protein for efficient entry of HIV-1 into cells.
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HIV-1 有效进入细胞所需的 Nef 蛋白的生产者细胞依赖性需求。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
A. Adachi
中科院分区：
文献类型：
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作者：
K. Tokunaga;A. Kojima;T. Kurata;K. Ikuta;R. Inubushi;R. Shimano;M. Kawamura;H. Akari;A. Koyama;A. Adachi
人类免疫缺陷病毒1型（HIV-1）的前病毒nef基因突变因其早期复制步骤缺陷而被评估。用野生型（wt）或nef突变克隆转染的6株cd4阳性和阴性细胞系制备病毒库，并将其接种到2株cd4阳性靶细胞系中，以监测病毒进入过程的效率。在一个细胞系中产生的nef突变病毒粒子在进入过程中表现出严重的缺陷，尽管在其他五个细胞系中产生的病毒粒子在进入细胞时的效率仅略低于wt病毒粒子。这些结果表明，HIV-1 Nef对病毒以依赖于产生细胞的方式有效进入至关重要。
A proviral nef gene mutant of human immunodeficiency virus type 1 (HIV-1) was evaluated for its defective early replication step. Virus stocks were prepared from six CD4-positive and -negative cell lines transfected with wild-type (wt) or the nef mutant clone and inoculated into two target CD4-positive cell lines to monitor the efficiency of viral entry process. The nef mutant virions produced in one cell line exhibited a severe defect in the entry process, although those produced in the other five cell lines were only slightly less efficient than the wt virions at entering into cells. These results have demonstrated that the HIV-1 Nef is critical for efficient viral entry in a producer cell-dependent manner.
nef等位基因对人类免疫缺陷病毒1型复制的影响。
影响因子：
3.7
作者：
Zazopoulos,E;Haseltine,WA
通讯作者：
Haseltine,WA