Spinal microglial motility is independent of neuronal activity and plasticity in adult mice.
Spinal microglial motility is independent of neuronal activity and plasticity in adult mice.
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DOI：
10.1186/1744-8069-6-19
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发表时间：
2010-04-09
期刊：
影响因子：
3.3
通讯作者：
Zhuo M
中科院分区：
文献类型：
--
作者：
Chen T;Koga K;Li XY;Zhuo M
小胶质细胞是驻留在中枢神经系统的巨噬细胞。在脊髓背角，小胶质细胞在生理感觉过程中处于静止状态，在周围神经损伤等病理条件下被激活。在这样的情况下，附近休息的小胶质细胞增加了它们的运动性，并在损伤部位积累。然而，支持神经活动可以增强小胶质细胞运动性的直接证据尚未见报道。在这项研究中，我们研究了在活体神经损伤下脊髓小胶质细胞的激活是否可以被脊髓切片制备中的神经元活动所模拟。我们发现，局部应用脊髓兴奋性神经递质，如谷氨酸和P物质，不会引起脊髓背角小胶质细胞运动性的任何变化。小胶质细胞的运动不太可能受到其他递质、神经调节剂和趋化因子的调节，因为类似的应用如GABA、5-羟色胺、去甲肾上腺素、卡巴胆碱、Fractalkine或IL没有产生任何明显的影响。此外，以伤害性强度对脊髓背根纤维进行低频或高频刺激并不能引起小胶质细胞突起的任何增强伸展或收缩。相比之下，局部应用三磷酸腺苷引发了脊髓背角小胶质细胞的快速而强劲的激活。我们的结果首次证明，神经损伤后脊髓小胶质细胞的激活不太可能完全是由于神经元的活动，非神经元因素可能是脊髓背角神经损伤相关的小胶质细胞激活的原因。
Microglia are the resident macrophages in the central nervous system. In the spinal cord dorsal horn, microglia stay in resting condition during physiological sensory processing, and are activated under pathological conditions such as peripheral nerve injury. In cases such as this, the nearby resting microglia increase their motility and accumulate at the site of injury. However, direct evidence to support that nerve activity can enhance the motility of microglia has not yet to be reported. In this study we investigated whether the activation of spinal microglia under in vivo nerve injury may be mimicked by neuronal activity in the spinal cord slice preparation. We found that local application of spinal excitatory neurotransmitters, such as glutamate and substance P did not cause any change in the motility of microglial cells in the spinal cord dorsal horn. The motility of microglial cells is unlikely modulated by other transmitters, neuromodulators and chemokines, because similar applications such as GABA, serotonin, noradrenaline, carbachol, fractalkine or interleukin did not produce any obvious effect. Furthermore, low or high frequency stimulation of spinal dorsal root fibers at noxious intensities failed to cause any enhanced extension or retraction of the microglia processes. By contrast, focal application of ATP triggered rapid and robust activation of microglial cells in the spinal dorsal horn. Our results provide the first evidence that the activation of microglia in the spinal cord after nerve injury is unlikely due solely to neuronal activity, non-neuronal factors are likely responsible for the activation of nerve injury-related microglial cells in the spinal dorsal horn.
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影响因子：
56.9
作者：
Nimmerjahn, A;Kirchhoff, F;Helmchen, F
通讯作者：
Helmchen, F
影响因子：
3.4
作者：
Calvo, CF;Cesselin, F;Glowinski, J
通讯作者：
Glowinski, J
DOI：
10.1073/pnas.95.18.10896
发表时间：
1998-09-01
影响因子：
11.1
作者：
Harrison, JK;Jiang, Y;Feng, LL
通讯作者：
Feng, LL
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR
影响因子：
56.9
作者：
Ikeda, Hiroshi;Stark, Johanna;Sandkuhler, Jurgen
通讯作者：
Sandkuhler, Jurgen