Apoptosis governs the elimination of Schistosoma japonicum from the non-permissive host Microtus fortis.
Apoptosis governs the elimination of Schistosoma japonicum from the non-permissive host Microtus fortis.
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DOI：
10.1371/journal.pone.0021109
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lin J
中科院分区：
文献类型：
--
作者：
Peng J;Gobert GN;Hong Y;Jiang W;Han H;McManus DP;Wang X;Liu J;Fu Z;Shi Y;Lin J
芦苇田鼠，东方田鼠，是唯一已知的哺乳动物宿主，其中日本血吸虫的血吸虫不能成熟，并引起显著发病。然而，关于日本血吸虫的成熟（因此，血吸虫病的发展）是如何在东方血吸虫中被阻止的，我们知之甚少。本研究首先采用扫描电镜和透射电镜对BALB/c小鼠和东方血吸虫感染10 d后的超微结构进行了比较。电镜观察发现，与BALB/c小鼠相比，东方血吸虫的被膜和亚被膜组织以及实质细胞的生长发育迟缓，超微结构存在差异。微阵列分析显示，两种鼠体血吸虫基因表达差异显著，前者血吸虫基因下调3293个（≥2倍），上调71个（≥2倍）。上调的基因包括一个编码颗粒蛋白（Grn）和原肌球蛋白的增殖相关基因。在来自东方血吸虫的血吸虫中下调的基因包括：编码杆状病毒IAP重复蛋白（SjIAP）和细胞因子诱导凋亡抑制剂（SjCIAP）的凋亡抑制基因，编码胰岛素代谢、长链脂肪酸代谢、信号转导、转化生长因子（TGF）途径、Wnt途径和发育相关分子的基因。TUNEL（末端脱氧核苷酸转移酶dUTP缺口末端标记）、PI/Annexin V-FITC检测、caspase 3/7活性分析和流式细胞术检测结果显示，与BALB/c小鼠相比，东阳血吸虫早期凋亡、晚期凋亡和/或坏死细胞的百分比以及caspase活性水平均显著高于BALB/c小鼠。
The reed vole, Microtus fortis, is the only known mammalian host in which schistosomes of Schistosoma japonicum are unable to mature and cause significant pathogenesis. However, little is known about how Schistosoma japonicum maturation (and, therefore, the development of schistosomiasis) is prevented in M. fortis. In the present study, the ultrastructure of 10 days post infection schistosomula from BALB/c mice and M. fortis were first compared using scanning electron microscopy and transmission electron microscopy. Electron microscopic investigations showed growth retardation and ultrastructural differences in the tegument and sub-tegumental tissues as well as in the parenchymal cells of schistosomula from M. fortis compared with those in BALB/c mice. Then, microarray analysis revealed significant differential expression between the schistosomula from the two rodents, with 3,293 down-regulated (by ≥2-fold) and 71 up-regulated (≥2 fold) genes in schistosomula from the former. The up-regulated genes included a proliferation-related gene encoding granulin (Grn) and tropomyosin. Genes that were down-regulated in schistosomula from M. fortis included apoptosis-inhibited genes encoding a baculoviral IAP repeat-containing protein (SjIAP) and cytokine-induced apoptosis inhibitor (SjCIAP), genes encoding molecules involved in insulin metabolism, long-chain fatty acid metabolism, signal transduction, the transforming growth factor (TGF) pathway, the Wnt pathway and in development. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) and PI/Annexin V-FITC assays, caspase 3/7 activity analysis, and flow cytometry revealed that the percentages of early apoptotic and late apoptotic and/or necrotic cells, as well as the level of caspase activity, in schistosomula from M. fortis were all significantly higher than in those from BALB/c mice.
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影响因子：
4.4
作者：
Gobert GN;Moertel L;Brindley PJ;McManus DP
通讯作者：
McManus DP
TGF-β信号传导控制寄生虫烟瘤曼森的胚胎发育。
影响因子：
6.7
作者：
Freitas TC;Jung E;Pearce EJ
通讯作者：
Pearce EJ
影响因子：
2.7
作者：
Dong, HF;Chen, XB;Jiang, MS
通讯作者：
Jiang, MS
Wnt4 是日本血吸虫中发现的 Wnt 家族的第一个成员，通过经典途径调节蠕虫发育
影响因子：
2
作者：
Li, Hong-Fei;Wang, Xiao-Bo;Lin, Jiao-Jiao
通讯作者：
Lin, Jiao-Jiao
影响因子：
2.1
作者：
Gobert, Geoffrey N.;McInnes, Russell;McManus, Donald P.
通讯作者：
McManus, Donald P.