Developmental gene expression profiles of the human pathogen Schistosoma japonicum.
Developmental gene expression profiles of the human pathogen Schistosoma japonicum.
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DOI：
10.1186/1471-2164-10-128
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发表时间：
2009-03-25
期刊：
影响因子：
4.4
通讯作者：
McManus DP
中科院分区：
文献类型：
--
作者：
Gobert GN;Moertel L;Brindley PJ;McManus DP
血吸虫血吸虫是一种复杂的吸虫，引起一种慢性寄生虫病，在世界范围内具有重要的公共卫生重要性，即血吸虫病。它们的生命周期具有明显的寄生和自由生活阶段，涉及哺乳动物和蜗牛宿主以及淡水。微阵列分析分析了亚洲种日本血吸虫的发育基因表达。总rna是从生命周期的三个不同环境阶段分离出来的-水生/蜗牛（卵，miracidia，孢子囊，尾蚴），幼虫（肺血吸虫和配对但未产卵的成虫）和成虫（配对，成熟雄虫和产卵雌虫，分别进行检测）。包括方差分析、主成分分析和层次聚类在内的高级分析提供了血吸虫不同发育阶段基因表达关系的全局概要。基因表达谱与吸虫的发育阶段在其生命周期中必须适应的主要环境设置有关。差异表达基因的基因本体揭示了广泛的功能和过程。此外，还发现了阶段特异性、差异表达的基因，这些基因参与了许多生物学途径和功能，包括钙信号、鞘脂代谢和寄生虫防御。这些发现提供了一个重要的人类病原体基因表达的综合数据库，包括在发育过程中参与逃避宿主免疫反应、营养获取、能量产生、钙信号传导、鞘脂代谢、产卵和被皮功能的基因的转录变化。这一资源应有助于确定和确定控制血吸虫病的新的抗血吸虫药物和疫苗目标的优先次序。
The schistosome blood flukes are complex trematodes and cause a chronic parasitic disease of significant public health importance worldwide, schistosomiasis. Their life cycle is characterised by distinct parasitic and free-living phases involving mammalian and snail hosts and freshwater. Microarray analysis was used to profile developmental gene expression in the Asian species, Schistosoma japonicum. Total RNAs were isolated from the three distinct environmental phases of the lifecycle – aquatic/snail (eggs, miracidia, sporocysts, cercariae), juvenile (lung schistosomula and paired but pre-egg laying adults) and adult (paired, mature males and egg-producing females, both examined separately). Advanced analyses including ANOVA, principal component analysis, and hierarchal clustering provided a global synopsis of gene expression relationships among the different developmental stages of the schistosome parasite. Gene expression profiles were linked to the major environmental settings through which the developmental stages of the fluke have to adapt during the course of its life cycle. Gene ontologies of the differentially expressed genes revealed a wide range of functions and processes. In addition, stage-specific, differentially expressed genes were identified that were involved in numerous biological pathways and functions including calcium signalling, sphingolipid metabolism and parasite defence. The findings provide a comprehensive database of gene expression in an important human pathogen, including transcriptional changes in genes involved in evasion of the host immune response, nutrient acquisition, energy production, calcium signalling, sphingolipid metabolism, egg production and tegumental function during development. This resource should help facilitate the identification and prioritization of new anti-schistosome drug and vaccine targets for the control of schistosomiasis.
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影响因子：
2.4
作者：
Bahia, D.;Avelar, L. G. A.;Mortara, R. A.
通讯作者：
Mortara, R. A.
DOI：
10.1016/s0169-4758(97)01205-2
发表时间：
1998-03-01
期刊：
PARASITOLOGY TODAY
影响因子：
--
作者：
Gobert, GN
通讯作者：
Gobert, GN
影响因子：
2.1
作者：
Gobert, Geoffrey N.;McInnes, Russell;McManus, Donald P.
通讯作者：
McManus, Donald P.
影响因子：
1.5
作者：
Fitzpatrick, JM;Johansen, MV;Hoffmann, KF
通讯作者：
Hoffmann, KF
影响因子：
1.5
作者：
Bickle, QD;Oldridge, J
通讯作者：
Oldridge, J