Effect of hyaluronic acid on bone formation in the expanded interpremaxillary suture in rats.
Effect of hyaluronic acid on bone formation in the expanded interpremaxillary suture in rats.
复制标题
透明质酸对大鼠扩张前颌间缝骨形成的影响。
DOI：
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发表时间：
2016
影响因子：
3.1
通讯作者：
N. Ulaş
中科院分区：
文献类型：
--
作者：
T. Sadıkoğlu;Didem Nalbantgil;Feyza Ulkur;N. Ulaş
目的
观察不同分子量透明质酸对大鼠颌间缝扩张后骨形成的影响。
材料和方法
将24只雄性Sprague道利大鼠分为3组。每组进行5天的扩增和10天的保留。第1组接受50 μl高分子量透明质酸（HMWHA），第2组接受50 μl低分子量透明质酸（LMWHA），对照组接受等量的生理盐水至颌间缝。注射后10天，处死大鼠并解剖其上颌骨。对于组织形态学评价，以10 μm间隔连续切片组织块。切片用苏木精-伊红（HE）染色，并用图像分析软件进行评价。评价骨面积（μm²）（BA）、骨缝边界周长（μm）（BP）以及成骨细胞和毛细血管细胞与BA和BP参数的比值。
结果
与LMWHA和对照组相比，HMWHA显示出统计学上更高的成骨细胞和毛细血管细胞评分比率（p < 0.05）。LMWHA组与对照组比较差异无统计学意义（p > 0.05）。
结论
在快速上颌骨扩张后，在颌间缝中局部注射HMWHA可刺激新骨形成，这可能会缩短保留期，并可能降低复发风险。LMWHA对颌间缝骨形成无影响。
OBJECTIVE
To evaluate the histomorphometric effects of different molecular weight hyaluronic acid on bone formation in rats after expansion of the interpremaxillary suture.
MATERIAL AND METHODS
Twenty-four male Sprague Dawley rats were divided into three groups. Each group was subjected to expansion for 5 days and retention for 10 days. Group 1 received 50 μl of high molecular weight hyaluronic acid (HMWHA), group 2 received 50 μl of low molecular weight hyaluronic acid (LMWHA), and the control group received same amount of saline solution to the interpremaxillary suture. Ten days after injection, the rats were killed and their maxillas dissected. For the histomorphometric evaluation, blocks were serially sectioned at 10-μm intervals. Sections were stained with hematoxylin-eosin (HE) and evaluated with image analysis software. Bone area (μm²) (BA), bone perimeter of suture borders (μm) (BP), and ratio of osteoblast cells and capillary cells to BA and BP parameters were evaluated.
RESULTS
HMWHA showed a statistically higher ratio of osteoblast and capillary cell scores compared with the LMWHA and control groups (p < 0.05). There were no statistically significant differences in between LMWHA and control groups (p > 0.05).
CONCLUSIONS
Local injection of HMWHA in the interpremaxillary suture after rapid maxillary expansion stimulated new bone formation, which may shorten the retention period and may reduce the risk of relapse. LMWHA has no effect on bone formation in interpremaxillary suture.
影响因子：
4.1
作者：
Hou, Bo;Fukai, Naomi;Olsen, Bjorn R.
通讯作者：
Olsen, Bjorn R.
影响因子：
2
作者：
WEIGEL, PH;FULLER, GM;LEBOEUF, RD
通讯作者：
LEBOEUF, RD