Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection.
Toll-like receptor 7-induced immune response to cutaneous West Nile virus infection.
复制标题
Toll样受体7诱导对皮肤西尼罗河病毒感染的免疫反应。
DOI：
10.1099/vir.0.011783-0
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发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Welte T;Reagan K;Fang H;Machain-Williams C;Zheng X;Mendell N;Chang GJ;Wu P;Blair CD;Wang T
The Toll-like receptor (TLR) 7 response represents a vital host defense mechanism in a murine model of systemic West Nile virus (WNV) infection. Here, we investigated the role of the TLR7-induced immune response following cutaneous WNV infection. We found that there was no difference in susceptibility to WNV encephalitis between wild-type and TLR7−/− mice upon intradermal injection or infected mosquito feeding. Viral load analysis revealed similar levels of WNV RNA in the peripheral tissues and brains of these two groups of mice following intradermal infection. There was a higher level of cytokines in the blood of wild-type mice at early stages of infection; however, this difference was diminished in the blood and brains at later stages. Langerhans cells (LCs) are permissive to WNV infection and migrate from the skin to draining lymph nodes upon intradermal challenge. Our data showed that WNV infection of TLR7−/− keratinocytes was significantly higher than wild-type keratinocytes. Infection of wild-type keratinocytes induced higher levels of IFN-α, IL-1β, IL-6 and IL-12, which might promote LC migration from the skin. Coculture of naive LCs of wild-type mice with WNV infected wild-type keratinocytes resulted in the production of more IL-6 and IL-12 than with TLR7−/− keratinocytes or by cultured LCs alone. Moreover, LCs in the epidermis were reduced in wild-type mice but not in TLR7−/− mice following intradermal WNV infection. Overall, our results suggest that the TLR7 response following cutaneous infection promotes LCs migration from the skin, which might compromise its protective effect in systemic infection.
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影响因子：
4.4
作者：
Kalali, Behnam Naderi;Koellisch, Gabriele;Ollert, Markus
通讯作者：
Ollert, Markus
影响因子：
3.7
作者：
Beasley, DWC;Li, L;Barrett, ADT
通讯作者：
Barrett, ADT
影响因子：
64.8
作者：
Alexopoulou, L;Holt, AC;Flavell, RA
通讯作者：
Flavell, RA
DOI：
10.1084/jem.184.2.741
发表时间：
1996-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Flacher, Vincent;Bouschbacher, Marielle;Valladeau, Jenny
通讯作者：
Valladeau, Jenny