CODON OPTIMIZATION OF THE POTENTIAL ANTIGENS ENCODING GENES FROM MYCOBACTERIUM TUBERCULOSIS
CODON OPTIMIZATION OF THE POTENTIAL ANTIGENS ENCODING GENES FROM MYCOBACTERIUM TUBERCULOSIS
复制标题
结核分枝杆菌潜在抗原编码基因的密码子优化
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Vijai Singh
中科院分区：
文献类型：
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作者：
I. Mani;D. Chaudhary;P. Somvanshi;Vijai Singh
Current approach for controlling of tuberculosis is going on by recommended doses of vaccines. Codon optimization and simulation techniques are used to improve the protein expression in living organism by increasing their translational efficiency of gene of interest. We have designed; optimized the codon and simulated in nineteen indigenous genes of Mycobacterium tuberculosis H37Rv in the Escherichia coli. We minimized the G+C content in optimized genes from 64.75% to 59.67% of the studied genes as the richness of G+C content is reflected in a strong bias. CAI and AT of optimized DNA were enhanced by 1.9 (47.8%) and 1.1 (12.5%) fold more with respect to its native type. Our finding indicates the optimized genes can be useful for over expression in host and the study provides a new insight for the emerging research in synthetic biology.
影响因子：
14.9
作者：
SHARP, PM;LI, WH
通讯作者：
LI, WH