Actin and Vimentin proteins with N-terminal deletion detected in tumor-bearing rat livers induced by intraportal-vein injection of Ha-ras-transfected rat liver cells.
Actin and Vimentin proteins with N-terminal deletion detected in tumor-bearing rat livers induced by intraportal-vein injection of Ha-ras-transfected rat liver cells.
复制标题
DOI：
10.1002/ijc.24229
复制
发表时间：
2009-06-01
影响因子：
6.4
通讯作者：
Sato, Kenji
中科院分区：
文献类型：
--
作者：
Nakamura, Yasushi;Kominami, Akari;Tsujimoto, Yoshiyuki;Nakayama, Yuko;Kitahashi, Tsukasa;Yoshimoto, Sonoko;Kubo, Asuka;Watanabe, Shinpei;Kageyama, Minami;Yokoyama, Meiko;Kido, Yasuhiro;Kobayashi, Yukiko;Kuwahata, Masashi;Chang, Chia-Cheng;Upham, Brad L.;Trosko, James E.;Park, Eun Young;Sato, Kenji
The introduction of the tumorigenic v-Ha-ras oncogene-transformed rat liver epithelial cells (WBras), which is deficient in gap junctional intercellular communication (GJIC), into F344 rats, induces significant formation of hepatocellular tumors. GJIC plays a major role in maintaining tissue homeostasis. Using this in vivo tumor model system, we used 2-dimensional electrophoresis with isoelectric focusing in the first dimension and SDS-PAGE in the second dimension to globally identify proteins that are uniquely expressed in the livers of WBras-treated rats as compared to the sham control. Immunoblotting was used to identify Ras and Connexin43, which were the positive and negative marker proteins, respectively, of the introduced WBras cells. As predicted, immunoblotting indicated that the whole liver of tumor-bearing animals exhibited a decreased level of Connexin43 and an increased level of Ras. Connexin43 and GJIC were expressed and functional in normal liver, but not in the tumor. In addition to these two markers, an additional four proteins exhibited decreased levels and two proteins exhibited increased levels in the livers of tumor bearing animals. N-Terminal sequencing analysis was used to identify these proteins which were glucose-regulated protein 78, two isoforms of heat shock protein 60, and the β-chain of ATP synthase for the down regulated proteins, and β-Actin with a 46 amino acid deletion from its N-terminus and Vimentin with a 71 amino acid deletion from its N-terminus for the up regulated proteins. These data offer potentially new markers of liver tumorigenicity, particularly Vimentin.
登录
查看更多内容
影响因子：
4.6
作者：
DEFEIJTER, AW;TROSKO, JE;LIEBERMAN, MW
通讯作者：
LIEBERMAN, MW
影响因子：
3.4
作者：
Fella, K;Glückmann, M;Kröger, M
通讯作者：
Kröger, M
影响因子：
4.7
作者：
HOSSAIN, MZ;WILKENS, LR;BERTRAM, JS
通讯作者：
BERTRAM, JS
影响因子：
4.7
作者：
Huard, C;Druesne, N;Chaumontet, C
通讯作者：
Chaumontet, C
影响因子：
9.7
作者：
Na, HK;Wilson, MR;Trosko, JE
通讯作者：
Trosko, JE