Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.
Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.
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嵌合具有感染性支气管炎病毒的峰值糖蛋白的嵌合病毒样颗粒的产生和免疫原性。
DOI：
10.4142/jvs.2014.15.2.209
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发表时间：
2014
影响因子：
1.8
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Lv L;Li X;Liu G;Li R;Liu Q;Shen H;Wang W;Xue C;Cao Y
传染性支气管炎病毒（IBV）是一种严重威胁养禽业的病毒，在世界范围内造成严重的经济损失。疫苗接种是预防传染病和控制传染病传播的最有效方法，但目前可用的灭活和减毒病毒疫苗存在一些缺点。我们开发了一种基于嵌合病毒样颗粒（VLP）的候选疫苗用于IBV保护。将H5 N1亚型禽流感病毒的基质1蛋白（matrix 1）与神经氨酸酶（NA）蛋白的胞浆区和跨膜区融合，得到融合蛋白NA/spike 1（S1）。嵌合VLP在肌内免疫的小鼠和鸡中引起比灭活IBV病毒显著更高的S1特异性抗体应答。此外，嵌合VLP在SPF鸡中诱导的中和抗体水平显著高于灭活H120病毒。最后，嵌合VLP在小鼠中诱导显著更高的IL-4产生。这些结果表明嵌合VLP具有用于针对IBV感染的疫苗的潜力。
Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV protection. The chimeric VLP was composed of matrix 1 protein from avian influenza H5N1 virus and a fusion protein neuraminidase (NA)/spike 1 (S1) that was generated by fusing IBV S1 protein to the cytoplasmic and transmembrane domains of NA protein of avian influenza H5N1 virus. The chimeric VLPs elicited significantly higher S1-specific antibody responses in intramuscularly immunized mice and chickens than inactivated IBV viruses. Furthermore, the chimeric VLPs induced significantly higher neutralization antibody levels than inactivated H120 virus in SPF chickens. Finally, the chimeric VLPs induced significantly higher IL-4 production in mice. These results demonstrate that chimeric VLPs have the potential for use in vaccines against IBV infection.
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影响因子：
5.5
作者：
Lokugamage KG;Yoshikawa-Iwata N;Ito N;Watts DM;Wyde PR;Wang N;Newman P;Kent Tseng CT;Peters CJ;Makino S
通讯作者：
Makino S
影响因子：
2.8
作者：
Gelb, J;Weisman, Y;Meir, R
通讯作者：
Meir, R
影响因子：
5.4
作者：
Arndt, Ariel L.;Larson, Blake J.;Hogue, Brenda G.
通讯作者：
Hogue, Brenda G.
影响因子：
5.4
作者：
Barman, S;Adhikary, L;Nayak, DP
通讯作者：
Nayak, DP
影响因子：
5.4
作者：
Murawski, Matthew R.;McGinnes, Lori W.;Morrison, Trudy G.
通讯作者：
Morrison, Trudy G.