Transient expression of cellular retinol‐binding protein‐1 during cardiac repair after myocardial infarction
Transient expression of cellular retinol‐binding protein‐1 during cardiac repair after myocardial infarction
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心肌梗死后心脏修复过程中细胞视黄醇结合蛋白-1的瞬时表达
DOI：
10.1111/j.1440-1827.2012.02802.x
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发表时间：
2012
影响因子：
2.2
通讯作者：
H. Hao
中科院分区：
文献类型：
--
作者：
Mengyue Yu;H. Ishibashi;Keiko Ohta;G. Gabbiani;M. Yamagishi;K. Hayashi;S. Hirota;M. Bochaton;H. Hao
维甲酸（RA）是一种具有多种生物学效应的维生素A衍生物。RA的细胞内转运和代谢受细胞视黄醇结合蛋白（CRBP）调节。CRBP-1在伤口愈合期间在肉芽组织成纤维细胞中瞬时表达;然而，其在心脏重塑中的作用仍然未知。结扎大鼠左冠状动脉建立心肌梗死（MI）模型，分别于术后3、6、15、30、45天取心脏。使用抗波形蛋白、抗α-平滑肌肌动蛋白（α-SMA）、抗基质金属蛋白酶（MMP）-2、抗MMP-9和抗CRBP-1抗体对心脏切片进行免疫组织化学检查。心肌梗死累及左心室48.8 ± 3.6%，并伴有重要的心脏重构。波形蛋白阳性成纤维细胞包括α-SMA阳性肌成纤维细胞在MI后3、6和15天表达CRBP-1。CRBP-1的表达在梗死后6天达到最大值。此后，CRBP-1表达显著降低，显示出与MMP表达相似的趋势。近期心肌梗死患者的人心脏标本通过免疫组织化学证实存在CRBP-1阳性成纤维细胞。我们已经证明CRBP-1在心脏重塑期间由成纤维细胞瞬时表达。我们的结果表明，CRBP-1据称通过其RA结合活性在MI后的心室重塑中发挥作用。
Retinoic acid (RA) is a vitamin A derivative that exerts pleiotropic biological effects. Intracellular transport and metabolism of RA are regulated by cellular retinol‐binding proteins (CRBP). CRBP‐1 is transiently expressed in granulation tissue fibroblasts during wound healing; however, its role in cardiac remodeling remains unknown. A rat myocardial infarction (MI) model was established by ligation of the left coronary artery, and hearts were obtained at 3, 6, 15, 30 and 45 days after operation. Heart sections were examined immunohistochemically using anti‐vimentin, anti‐α‐smooth muscle actin (α‐SMA), anti‐matrix metalloproteinase (MMP)‐2, anti‐MMP‐9 and anti‐CRBP‐1 antibodies. Infarction involved 48.8 ± 3.6% of the left ventricle and was followed by an important cardiac remodeling. Vimentin‐positive fibroblastic cells including α‐SMA‐positive myofibroblasts expressed CRBP‐1 at 3‐, 6‐, and 15‐days after MI. Expression of CRBP‐1 reached a maximum at 6‐days after infarction. Thereafter, CRBP‐1 expression was dramatically decreased, showing a similar tendency to MMP expression. Human heart specimens of individuals with a recent myocardial infarction demonstrated presence of CRBP‐1‐positive fibroblasts by immunohistochemistry. We have demonstrated that CRBP‐1 is transiently expressed by fibroblasts during cardiac remodeling. Our results suggest that CRBP‐1 plays a role in ventricular remodeling after MI allegedly through its RA binding activity.
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DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ong,DE;MacDonald,PN;Gubitosi,AM
通讯作者：
Gubitosi,AM
细胞视黄醇结合蛋白和细胞视黄酸结合蛋白在大鼠睾丸和附睾中的定位。
影响因子：
--
作者：
Porter,SB;Ong,DE;Chytil,F;Orgebin-Crist,MC
通讯作者：
Orgebin-Crist,MC
影响因子：
6.1
作者：
ONG, DE
通讯作者：
ONG, DE
不依赖胆酸的视黄酯水解。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Boerman,MH;Napoli,JL
通讯作者：
Napoli,JL
类视黄醇调节内皮细胞产生基质降解蛋白酶和金属蛋白酶组织抑制剂 (TIMP)。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Braunhut,SJ;Moses,MA
通讯作者：
Moses,MA