Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen.
Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen.
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DOI：
10.1016/j.joca.2012.06.002
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发表时间：
2012-10
影响因子：
7
通讯作者：
Prockop, D. J.
中科院分区：
文献类型：
--
作者：
Horie, M.;Choi, H.;Lee, R. H.;Reger, R. L.;Ylostalo, J.;Muneta, T.;Sekiya, I.;Prockop, D. J.
Meniscal regeneration was previously shown to be enhanced by injection of mesenchymal stem/stromal cells (MSCs) but the mode of action of the MSCs was not established. The aim of this study was to define how injection of MSCs enhances meniscal regeneration. A hemi-meniscectomy model in rats was used. Rat-MSCs (rMSCs) or human-MSCs (hMSCs) were injected into the right knee joint after the surgery, and PBS was injected into the left. The groups were compared macroscopically and histologically at 2, 4, and 8 w. The changes in transcription in both human and rat genes were assayed by species-specific microarrays and real-time RT-PCRs. Although the number of hMSCs decreased with time, hMSCs enhanced meniscal regeneration in a manner similar to rMSCs. hMSCs injection increased expression of rat type II collagen (rat-Col II), and inhibited osteoarthritis progression. The small fraction of hMSCs were activated to express high levels of a series of genes including Indian hedgehog (Ihh), parathyroid hormone-like hormone (PTHLH), and bone morphogenetic protein 2 (BMP2). The presence of hMSCs triggered the subsequent expression of rat-Col II. An antagonist of hedgehog signaling inhibited the expression of rat-Col II and an agonist increased expression of rat-Col II in the absence of hMSCs. Despite rapid reduction in cell numbers, intra-articular injected hMSCs were activated to express Ihh, PTHLH, and BMP2 and contributed to meniscal regeneration. The hedgehog signaling was essential in enhancing the expression of rat-Col II, but several other factors provided by the hMSCs probably contributed to the repair.
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影响因子：
3.8
作者：
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通讯作者：
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影响因子：
4
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Longaker, Michael T.
影响因子：
4.8
作者：
CANNON, WD;VITTORI, JM
通讯作者：
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影响因子：
7.8
作者：
Amling, M;Neff, L;Tanaka, S;Inoue, D;Kuida, K;Weir, E;Philbrick, WM;Broadus, AE;Baron, R
通讯作者：
Baron, R
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4
作者：
Caplan, Arnold I.;Dennis, James E.
通讯作者：
Dennis, James E.