Phycobilisomes supply excitations to both photosystems in a megacomplex in cyanobacteria.

Phycobilisomes supply excitations to both photosystems in a megacomplex in cyanobacteria.
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DOI:
10.1126/science.1242321
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发表时间:
2013-11-29
期刊:
Science (New York, N.Y.)
影响因子:
--
通讯作者:
Blankenship RE
Blankenship RE
中科院分区:
其他
文献类型:
--
作者:
Liu H;Zhang H;Niedzwiedzki DM;Prado M;He G;Gross ML;Blankenship RE

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In photosynthetic organisms, photons are captured by light-harvesting antenna complexes, and energy is transferred to reaction centers where photochemical reactions take place. We describe here the isolation and characterization of a fully functional megacomplex composed of a phycobilisome antenna complex and photosystems I and II from the cyanobacterium Synechocystis PCC 6803. A combination of in vivo protein cross-linking, mass spectrometry, and time-resolved spectroscopy indicates that the megacomplex is organized to facilitate energy transfer but not intercomplex electron transfer, which requires diffusible intermediates and the cytochrome b6f complex. The organization provides a basis for understanding how phycobilisomes transfer excitation energy to reaction centers and how the energy balance of two photosystems is achieved, allowing the organism to adapt to varying ecophysiological conditions.
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