Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor.
Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor.
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DOI：
10.1111/nph.14176
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Dodd AN
中科院分区：
文献类型：
--
作者：
Belbin FE;Noordally ZB;Wetherill SJ;Atkins KA;Franklin KA;Dodd AN
我们研究了调节叶绿体转录响应环境信号的信号通路。控制质体转录的一种机制涉及质体编码的质体RNA聚合酶的核编码σ亚基。编码sigma因子SIG 5的转录本受光和生物钟调节。然而，SIG 5的叶绿体靶标受SIG 5表达的光诱导变化调节的程度是未知的。此外，光感受器信号转导途径的生理节律调节叶绿体转录的SIG 5是未知的。我们监测了拟南芥光感受器和sigma因子突变体中叶绿体转录的调控。我们建立了一个叶绿体的转录响应光强度介导的SIG 5;叶绿体的转录响应的相对比例的红色和远红光的SIG 5通过光敏色素和光合信号进行调节;和昼夜节律调节的叶绿体转录SIG 5主要依赖于蓝光和隐花色素。我们的实验揭示了一个单一的σ因子调节叶绿体转录的光环境信号的广泛整合。这可能源于一种进化上古老的机制，该机制保护光合细菌免受强光胁迫，随后与高等植物光转导网络整合。
We investigated the signalling pathways that regulate chloroplast transcription in response to environmental signals. One mechanism controlling plastid transcription involves nuclear‐encoded sigma subunits of plastid‐encoded plastid RNA polymerase. Transcripts encoding the sigma factor SIG5 are regulated by light and the circadian clock. However, the extent to which a chloroplast target of SIG5 is regulated by light‐induced changes in SIG5 expression is unknown. Moreover, the photoreceptor signalling pathways underlying the circadian regulation of chloroplast transcription by SIG5 are unidentified. We monitored the regulation of chloroplast transcription in photoreceptor and sigma factor mutants under controlled light regimes in Arabidopsis thaliana. We established that a chloroplast transcriptional response to light intensity was mediated by SIG5; a chloroplast transcriptional response to the relative proportions of red and far red light was regulated by SIG5 through phytochrome and photosynthetic signals; and the circadian regulation of chloroplast transcription by SIG5 was predominantly dependent on blue light and cryptochrome. Our experiments reveal the extensive integration of signals concerning the light environment by a single sigma factor to regulate chloroplast transcription. This may originate from an evolutionarily ancient mechanism that protects photosynthetic bacteria from high light stress, which subsequently became integrated with higher plant phototransduction networks.
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影响因子：
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作者：
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DOI：
10.1073/pnas.1221738110
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