Developmental expression of the Xenopus int-2 (FGF-3) gene: activation by mesodermal and neural induction.
Developmental expression of the Xenopus int-2 (FGF-3) gene: activation by mesodermal and neural induction.
复制标题
非洲爪蟾 int-2 (FGF-3) 基因的发育表达：通过中胚层和神经诱导激活。
作者：
D. Tannahill;H. Isaacs;M. Close;G. Peters;J. Slack
我们使用了一种针对爪蟾同源的哺乳动物原癌基因int-2 （FGF-3）特异性探针来检测该基因在爪蟾发育过程中的时空表达模式。从原肠胚开始到幼虫前期，Int-2都在表达。在早期原肠胚中，它在胚孔唇周围表达。这在神经鞘的准中胚层和神经外胚层的后三分之一处保持。仅在神经外胚层前三分之一的第二个表达域出现在原肠末，随后分解到视囊、下丘脑和中脑-后脑连接区。在尾芽至前幼虫胚胎中还存在其他表达域，包括气孔间充质、咽囊的内胚层和耳囊两侧的颅神经节。结果表明，用bFGF和激活素处理囊胚外胚层，可诱导表达int-2。通过将原肠胚外胚层异型移植到蝾螈神经板中，我们也证实了int-2可以在神经诱导下表达。这些结果表明，int-2在发育过程中具有多种功能，包括早期在体前后轴的形成中起作用，后来在尾巴、脑源性结构和其他上皮的发育中起作用。
We have used a probe specific for the Xenopus homologue of the mammalian proto-oncogene int-2 (FGF-3) to examine the temporal and spatial expression pattern of the gene during Xenopus development. int-2 is expressed from just before the onset of gastrulation through to prelarval stages. In the early gastrula, it is expressed around the blastopore lip. This is maintained in the posterior third of the prospective mesoderm and neuroectoderm in the neurula. A second expression domain in the anterior third of the neuroectoderm alone appears in the late gastrula, which later resolves into the optic vesicles, hypothalamus and midbrain-hindbrain junction region. Further domains of expression arise in tailbud to prelarval embryos, including the stomodeal mesenchyme, the endoderm of the pharyngeal pouches and the cranial ganglia flanking the otocyst. It is shown, by treatment of blastula ectoderm with bFGF and activin, that int-2 can be expressed in response to mesoderm induction. By heterotypic grafting of gastrula ectoderm into axolotl neural plate, we have also demonstrated that int-2 can be expressed in response to neural induction. These results suggest that int-2 has multiple functions in development, including an early role in patterning of the anteroposterior body axis and a later role in the development of the tail, brain-derived structures and other epithelia.
影响因子：
4.6
作者：
L. Niswander;G. Martin
通讯作者：
L. Niswander;G. Martin