The byssus of the zebra mussel, Dreissena polymorpha. II: Structure and polymorphism of byssal polyphenolic protein families.

The byssus of the zebra mussel, Dreissena polymorpha. II: Structure and polymorphism of byssal polyphenolic protein families.
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斑马贻贝 (Dreissena polymorpha) 的足丝。

DOI:
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发表时间:
1993
期刊:
Molecular Marine Biology and Biotechnology
影响因子:
--
通讯作者:
Waite Jh
Waite Jh
中科院分区:
--
文献类型:
--
作者:
Rzepecki Lm;Waite Jh

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斑马贻贝 Dreissena polymorpha 的足丝是其粘附策略的关键要素。它由一束线组成,尖端有粘性斑块,并附着在贻贝足丝合成器官(脚)的底部。足丝的两种多酚蛋白前体已从足部纯化。这些前体 Dpfp-1 和 Dpfp-2(Dreissena polymorpha 脚蛋白)的表观 M(r) 值分别为 76 和 26 K,包含整合到其一级序列中的 3,4-二羟基苯丙氨酸 (DOPA),但与之前表征的来自海洋贻贝的多酚蛋白不同。相关的斑驴贻贝 (Dreissena spp.?) 具有同源蛋白质,但组成却显着不同。斑马贻贝多巴蛋白具有独特的寡肽基序序列串联重复,含有色氨酸,并且主要在苏氨酸残基上进行 O-糖基化。半乳糖胺是水解后检测到的唯一碳水化合物。 Dpfp-1 构成了一个多态性多肽家族,其 pI 值的酸性范围异常在 5.3 至 6.5 之间。线中以及线与斑块之间的接合处碳水化合物的检测表明,这两种蛋白质位于它们可能充当漆或结构元件的区域。
The byssus of the zebra mussel Dreissena polymorpha is the key element of its adhesive strategy. It consists of a bundle of threads tipped by adhesive plaques and attached to the mussel at the base of its byssal-synthesizing organ, the foot. Two polyphenolic protein precursors of the byssus have been purified from the foot. These precursors, Dpfp-1 and Dpfp-2 (Dreissena polymorpha foot protein), with apparent M(r) values of 76 and 26 K, respectively, contain 3,4-dihydroxyphenylalanine (DOPA) integrated into their primary sequence, but differ from previously characterized polyphenolic proteins from marine mussels. The related quagga mussel (Dreissena spp.?) has homologous proteins with significantly different compositions. The zebra mussel DOPA proteins are tandemly repetitive with unique oligopeptide motif sequences, contain tryptophan, and are O-glycosylated primarily on threonine residues. Galactosamine was the only carbohydrate detected after hydrolysis. Dpfp-1 constitutes a polymorphic family of polypeptides with, unusually, an acidic range of pI values between 5.3 and 6.5. The detection of carbohydrate in the thread and in the juncture between thread and plaque suggests that these two proteins are localized in those regions where they may function as lacquers or structural elements.
DOI: 10.1073/pnas.90.15.7144
发表时间: 1993-08
影响因子: 11.1
作者:
M. Verma;E. Davidson
通讯作者: M. Verma;E. Davidson
贻贝粘附蛋白成分膜的表面特性。
DOI: 10.1016/0142-9612(92)90150-m
发表时间: 1992
期刊: Biomaterials
影响因子: 14
作者:
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一些海洋双壳类软体动物足丝中的蛋白质梯度。
DOI: 10.1002/jez.1402400102
发表时间: 1986
期刊: The Journal of experimental zoology
影响因子: --
作者:
Mascolo,JM;Waite,JH
通讯作者: Waite,JH
DOI: --
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
Waite,JH
通讯作者: Waite,JH
特定肽序列的苏氨酸残基上的 α-N-乙酰半乳糖胺基化可在人纤连蛋白中产生癌胎肽表位。
DOI: --
发表时间: 1989
期刊: The Journal of biological chemistry
影响因子: --
作者:
Matsuura,H;Greene,T;Hakomori,S
通讯作者: Hakomori,S