A tilting device for three‐dimensional microscopy: Application to in situ imaging of interphase cell nuclei
A tilting device for three‐dimensional microscopy: Application to in situ imaging of interphase cell nuclei
复制标题
三维显微镜倾斜装置：在间期细胞核原位成像中的应用
作者：
J. Bradl;M. Hausmann;V. Ehemann;D. Komitowski;C. Cremer
光学显微镜在光轴(Z)方向上的分辨率比在焦平面(x-y平面)上的分辨率低得多。常规显微镜和共焦显微镜都是如此。对于定量显微镜，例如研究人类间期细胞核中染色体的三维组织，必须在仔细考虑成像系统的特性的情况下，通过点扩散函数或光学传递函数来重建三维图像。为了缓解重建问题，研制了一种倾斜装置，使同一细胞核在不同视图下的几个数据集可以配准。使用蔡司透射式光学显微镜Axiomat，利用计算机控制的步进电机沿z轴移动的工作台，从一系列光学切片中获得三维信息。Axiomat工作台上的倾斜装置可以使细胞核旋转任何所需的角度，还可以在x-y方向上移动。
The resolution of an optical microscope is considerably less in the direction of the optical axis (z) than in the focal plane (x‐y plane). This is true of conventional as well as confocal microscopes. For quantitative microscopy, for instance studies of the three‐dimensional (3‐D) organization of chromosomes in human interphase cell nuclei, the 3‐D image must be reconstructed by a point spread function or an optical transfer function with careful consideration of the properties of the imaging system. To alleviate the reconstruction problem, a tilting device was developed so that several data sets of the same cell nucleus under different views could be registered. The 3‐D information was obtained from a series of optical sections with a Zeiss transmission light microscope Axiomat using a stage with a computer‐controlled stepping motor for movement in the z‐axis. The tilting device on the Axiomat stage could turn a cell nucleus through any desired angle and also provide movement in the x‐y direction.
共焦显微镜：概述。
影响因子：
2.7
作者：
Shuman,H;Murray,JM;DiLullo,C
通讯作者：
DiLullo,C
间期细胞核中 DNA 序列的接近度与基因组距离相关，并允许跨 250 kilobase 对的粘粒进行排序。
影响因子：
4.4
作者：
Trask,B;Pinkel,D;vandenEngh,G
通讯作者：
vandenEngh,G
DOI：
10.1073/pnas.85.23.9138
发表时间：
1988-12-01
影响因子：
11.1
作者：
PINKEL, D;LANDEGENT, J;GRAY, J
通讯作者：
GRAY, J