Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.)
Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.)
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梨（Pyrus spp.）染色体的荧光染色分析
DOI：
10.2503/jjshs1.79.23
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发表时间：
2010
影响因子：
--
通讯作者：
S. Tominaga
中科院分区：
文献类型：
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作者：
Masashi Yamamoto;N. Takada;T. Hirabayashi;Kubo Tatsuya;S. Tominaga
从梨（Pyrus pyrifolia, Burm.） 3个品种的开放授粉幼苗的根尖上提取样品，测定了梨染色体的荧光带型。F) Nakai（日本梨），P. communis L.（欧洲梨）和P. bretschneideri Rehder（中国梨）]。根尖用2 mM 8-羟基喹啉在10℃下预处理4 h；采用酶浸风干法制备染色体样品。本研究使用的所有品种均有2n = 34条染色体。在4条染色体的端粒位置均观察到CMA阳性带。在一些样品中，这些CMA+波段是在卫星位置观测到的。4'-6-二氨基-2-苯基吲哚（DAPI）负带（−）与CMA+带对应。30条染色体无CMA+/DAPI -带。在所有染色体中未观察到碘化丙啶（PI）带。CMA+带的数量和位置较为稳定，3种间无明显差异。
Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of open-pollinated seedlings of six cultivars from three species [Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. communis L. (European pear), and P. bretschneideri Rehder (Chinese pear)]. Root tips were pretreated in 2 mM 8-hydroxyquinoline at 10°C for 4 h; the chromosome samples were prepared by the enzymatic maceration and air-drying method. All cultivars used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA) positive (+) bands were observed in telomeric positions of four chromosomes. In some samples, these CMA+bands were observed at satellite positions. 4'-6-diamidino-2-phenylindole (DAPI) negative bands (−) were seen to correspond with CMA+bands. Thirty chromosomes had no CMA+/DAPI−bands. No propidium iodide (PI) bands were observed in any chromosomes. The number and positions of CMA+bands were stable, and there was no difference among the three species.
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
Masashi Yamamoto
通讯作者：
Masashi Yamamoto