Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids.
Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids.
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DOI：
10.1016/j.biomaterials.2009.02.047
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发表时间：
2009-06
期刊：
影响因子：
14
通讯作者：
Takayama, Shuichi
中科院分区：
文献类型：
--
作者：
Hsiao, Amy Y.;Torisawa, Yu-suke;Tung, Yi-Chung;Sud, Sudha;Taichman, Russell S.;Pienta, Kenneth J.;Takayama, Shuichi
癌细胞所处的微环境对癌症的生长起着重要作用。因此，为了更好地了解癌症和促进治疗的发展，在体外模拟体内的肿瘤生态位是势在必行的。在这里，我们设计了一个3D转移性前列腺癌模型，其中包括转移性前列腺癌细胞所在的骨骼微环境中周围细胞的类型。具体地说，我们使用两层微流控系统培养了荧光标记的转移性前列腺癌细胞(PC-3细胞系)、成骨细胞和内皮细胞的3D多细胞型球体。这种方法确保了所有共培养细胞类型均匀地结合到每个球体中，并使球体保持不变，以便在至少一周的时间内跟踪单个球体和驻留在其中的PC-3‘S。该培养体系在不降低细胞存活率的情况下，大大降低了PC-3细胞的增殖率，可以更真实地再现恶性癌细胞在骨转移前列腺癌微环境中的体内生长行为。
The niche microenvironment in which cancer cells reside plays a prominent role in the growth of cancer. It is therefore imperative to mimic the in vivo tumor niche in vitro to better understand cancer and enhance development of therapeutics. Here, we engineer a 3D metastatic prostate cancer model that includes the types of surrounding cells in the bone microenvironment that the metastatic prostate cancer cells reside in. Specifically, we used a two-layer microfluidic system to culture 3D multi-cell type spheroids of fluorescently labeled metastatic prostate cancer cells (PC-3 cell line), osteoblasts and endothelial cells. This method ensures uniform incorporation of all co-culture cell types into each spheroid and keeps the spheroids stationary for easy tracking of individual spheroids and the PC-3's residing inside them over the course of at least a week. This culture system greatly decreased the proliferation rate of PC-3 cells without reducing viability and may more faithfully recapitulate the in vivo growth behavior of malignant cancer cells within the bone metastatic prostate cancer microenvironment.
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影响因子：
4.8
作者：
Korff, T;Kimmina, S;Augustin, HG
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0610868104
发表时间：
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影响因子：
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作者：
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作者：
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