Partitioning of gene expression among zebrafish photoreceptor subtypes.
Partitioning of gene expression among zebrafish photoreceptor subtypes.
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DOI：
10.1038/s41598-021-96837-z
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发表时间：
2021-08-30
影响因子：
4.6
通讯作者：
Corbo JC
中科院分区：
文献类型：
--
作者：
Ogawa Y;Corbo JC
脊椎动物的感光细胞分为两大类：视杆细胞和视锥细胞，分别负责暗光和亮光视觉。虽然区分视杆细胞和视锥细胞的许多分子特征是已知的，但视锥细胞亚型之间的基因表达差异仍然知之甚少。硬骨鱼以其感光系统的多样性而闻名。在这里，我们使用单细胞 RNA 测序来分析斑马鱼（一种硬骨鱼）的成年光感受器。我们发现，除了四种典型的斑马鱼视锥细胞类型外，还存在表达红移视蛋白旁系同源物（opn1mw4 或 opn1lw1）的绿色和红色视锥细胞亚群（之前显示位于腹侧视网膜）以及视锥细胞光转导基因的独特组合。此外，许多旁系同源光转导基因的表达在视锥细胞亚型之间分配，类似于脊椎动物中所见的光转导旁系同源物在视杆细胞和视锥细胞之间的分配。分离的锥体基因对是通过硬骨鱼特异性全基因组复制或后来的进化枝特异性基因复制产生的。我们还发现视锥细胞亚型表达不同的转录调节因子，包括许多以前未涉及光感受器发育或分化的因子。总的来说，我们的工作表明，通过差异表达转录调节因子的作用来划分旁系同源基因表达，使得硬骨鱼中视锥细胞亚型多样化。
Vertebrate photoreceptors are categorized into two broad classes, rods and cones, responsible for dim- and bright-light vision, respectively. While many molecular features that distinguish rods and cones are known, gene expression differences among cone subtypes remain poorly understood. Teleost fishes are renowned for the diversity of their photoreceptor systems. Here, we used single-cell RNA-seq to profile adult photoreceptors in zebrafish, a teleost. We found that in addition to the four canonical zebrafish cone types, there exist subpopulations of green and red cones (previously shown to be located in the ventral retina) that express red-shifted opsin paralogs (opn1mw4 or opn1lw1) as well as a unique combination of cone phototransduction genes. Furthermore, the expression of many paralogous phototransduction genes is partitioned among cone subtypes, analogous to the partitioning of the phototransduction paralogs between rods and cones seen across vertebrates. The partitioned cone-gene pairs arose via the teleost-specific whole-genome duplication or later clade-specific gene duplications. We also discovered that cone subtypes express distinct transcriptional regulators, including many factors not previously implicated in photoreceptor development or differentiation. Overall, our work suggests that partitioning of paralogous gene expression via the action of differentially expressed transcriptional regulators enables diversification of cone subtypes in teleosts.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
DOI：
10.1073/pnas.0902425106
发表时间：
2009-10-13
影响因子：
11.1
作者：
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通讯作者：
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作者：
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通讯作者：
Cepko, CL
影响因子：
13.6
作者：
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通讯作者：
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影响因子：
4.6
作者：
Kimura, Yukiko;Satou, Chie;Higashijima, Shin-ichi
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