Dopamine Depletion Preferentially Impairs D1 over D2‐Receptor Regulation of Striatal In Vivo Acetylcholine Release
Dopamine Depletion Preferentially Impairs D1 over D2‐Receptor Regulation of Striatal In Vivo Acetylcholine Release
复制标题
多巴胺耗竭优先损害 D1 而非 D2 受体对纹状体体内乙酰胆碱释放的调节
作者：
R. Bertorelli;M. Zambelli;G. Chiara;S. Consolo
用α-甲基-对-酪氨酸（a-methyl-p-tyrosine，A）急性（2 h）或长期（16 h）耗竭脑多巴胺（DA），观察120 min内D2和D1多巴胺能受体对纹状体乙酰胆碱（acetylcholine，ACh）释放的调节作用。DA传输的减少不影响基础ACh输出后2小时，但显着降低ACh释放16小时（50%）。急性α-甲基-p-酪氨酸预处理阻止了D1拮抗剂SCH 23390引起的ACh释放减少和D2拮抗剂瑞莫西必利引起的ACh释放增加，这与两种DA受体的DA传递急剧减少一致。然而，α-甲基-对-酪氨酸给药后16 h，Remoxipride对ACh释放的影响恢复，但SCH 23390仍无影响，表明D2对ACh释放的抑制作用恢复，而D1易化作用的降低持续存在。因此，D1对ACh神经锡永的易化控制似乎比D2抑制控制对DA传递减少更敏感。从这些数据中产生的DA-ACh相互作用的新模型为DA传递变化与锥体外系运动功能之间的关系提供了新的视角。
The roles of D2 and D1 dopaminergic receptors on the regulation of striatal acetylcholine (ACh) release in vivo were examined for a period of 120 min after acute (2 h) or prolonged (16 h) depletion of brain dopamine (DA) by a‐methyl‐p‐tyrosine. The reduction of DA transmission did not affect basal ACh output after 2 h but markedly lowered ACh release by 16 h (50%). Acute α‐methyl‐p‐tyrosine pre‐treatment prevented the reduction of ACh release by the D, antagonist SCH 23390 and its increase by the D2 antagonist, remoxipride, consistent with a drastic reduction of DA transmission at both DA receptors. However, 16 h after α‐methyl‐p‐tyrosine, the effect of remoxipride on ACh release was restored, but SCH 23390 still had no effect, suggesting that the D2 inhibitory tone on ACh release had recovered, whereas the reduction of the D1 facilitatory influence persisted. The D1 facilitatory control of ACh neurotransmis‐sion thus appears to be more sensitive than the D2 inhibitory control to a reduction in DA transmission. The new model of DA‐ACh interaction resulting from these data casts fresh light on the relationship between changes in DA transmission and extrapyramidal motor function.
选择性胆碱能神经毒素：AF64A 对大鼠纹状体的影响。
影响因子：
2.9
作者：
Sandberg,K;Hanin,I;Fisher,A;Coyle,JT
通讯作者：
Coyle,JT
DOI：
10.1073/pnas.88.5.1859
发表时间：
1991-03-01
影响因子：
11.1
作者：
WEINER, DM;LEVEY, AI;BRANN, MR
通讯作者：
BRANN, MR