A CIRCULAR-DICHROISM STUDY OF THE ENTEROTOXIN FROM CLOSTRIDIUM PERFRINGENS TYPE A
A CIRCULAR-DICHROISM STUDY OF THE ENTEROTOXIN FROM CLOSTRIDIUM PERFRINGENS TYPE A
复制标题
A型产气荚膜梭菌肠毒素的圆二色性研究
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
O. Harbitz
中科院分区：
文献类型：
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作者：
P. E. Granum;O. Harbitz
记录了A型产气荚膜梭菌肠毒素在不同条件下的远紫外圆二色谱，并进行了构象分析。天然肠毒素(pH 6.8)含有约80%的褶皱片层，20%的无规卷曲，无螺旋结构。通过加入少量的洗涤剂十二烷基硫酸钠(0-261mol十二烷基硫酸钠/摩尔肠毒素)，β-Sheet结构可以逐渐转变为更多的α-螺旋结构。8M尿素处理使肠毒素的二级结构发生了有限的变化，而热处理(60℃，30min)、6.5M盐酸胍或0.01M氢氧化钠处理则使无规卷曲的数量显著增加。
Far-UV circular-dichroism spectra of the enterotoxin from Clostridium perfringens type A were recorded under different conditions, and conformational analysis was performed. The native enterotoxin (pH 6.8) was shown to contain about 80% pleated sheet, 20% random coil and no helix structure. By adding small amounts of the detergent sodium dodecyl sulfate (0–261 mol SDS/mol enterotoxin), the β-sheet structure could gradually be changed to a more α-helical structure. Treatment with 8 M urea resulted in limited alterations of the secondary structure of the enterotoxin, while heat treatment (60°C, 30 min), treatment with 6.5 M guanidine hydrochloride or 0.01 M NaOH led to a dramatic increase in the amount of random coil.
鉴定来自兔刷状缘膜的 50,000 Mr 蛋白，该蛋白与产气荚膜梭菌肠毒素结合。
DOI：
10.1016/0006-291x(83)91731-x
发表时间：
1983
影响因子：
3.1
作者：
Wnek,AP;McClane,BA
通讯作者：
McClane,BA