Isolation and characterization of a fatty acid binding protein in adipose tissue of Gallus domesticus.

Isolation and characterization of a fatty acid binding protein in adipose tissue of Gallus domesticus.
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家鸡脂肪组织中脂肪酸结合蛋白的分离和表征。

DOI:
10.1016/0305-0491(90)90061-w
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发表时间:
1990
期刊:
Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子:
--
通讯作者:
P. Hargis
P. Hargis
中科院分区:
--
文献类型:
--
作者:
G. H. Sams;B. Hargis;P. Hargis

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1.脂肪酸结合蛋白(A-FABP)是从鸡脂肪胞液中分离得到的一种蛋白质。2.相对摩尔数从SDS-聚丙烯酰胺电泳确定鸡A-FABP的wt为14,400; pI为5.1;氨基酸组成数据表明与哺乳动物心脏和脂肪FABP结构同源。3.制备的抗A-FABP多克隆抗体对鸡A-FABP具有单特异性,与鸡肝蛋白无交叉反应。4.相对配体结合特性的测定表明,A-FABP表现出最大的结合活性,在响应亚油酸酯,其次是油酸酯,棕榈酰CoA和棕榈酸酯;没有检测到胆固醇的结合亲和力。
1. Fatty acid binding protein (A-FABP) was isolated from chicken adipose cytosol. 2. Relative mol. wt of chicken A-FABP was determined to be 14,400 from SDS-polyacrylamide electrophoresis; the pI was 5.1; and amino acid composition data indicated structural homology with mammalian heart and adipose FABPs. 3. Polyclonal antisera prepared against A-FABP exhibited monospecificity for chicken A-FABP and no cross-reactivity with chicken liver proteins was observed. 4. Determination of relative ligand binding characteristics indicated A-FABP exhibited greatest binding activity in response to linoleate, followed by oleate, palmityl CoA and palmitate; no binding affinity for cholesterol was detected.
大鼠和人脂肪组织中脂肪酸结合蛋白的表征和纯化。
DOI: 10.1016/0005-2760(82)90236-3
发表时间: 1982
期刊: Biochimica et biophysica acta
影响因子: --
作者:
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通讯作者: Shrago,E
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发表时间: 1988-05-01
影响因子: 4.1
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通讯作者: GRIGOR, MR
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DOI: --
发表时间: 1986
期刊: The Journal of biological chemistry
影响因子: --
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发表时间: 1985-03
期刊: The Journal of biological chemistry
影响因子: --
作者:
Lgwrence ChanSQ;Chik-Fong WeiSQ;Wen-Hsiung Lin;Chao-Yuh YangQ;Paul RatnerSQ;Henry PownallQ;Antonio M. Gotto;Louis C. SmithSQII
通讯作者: Lgwrence ChanSQ;Chik-Fong WeiSQ;Wen-Hsiung Lin;Chao-Yuh YangQ;Paul RatnerSQ;Henry PownallQ;Antonio M. Gotto;Louis C. SmithSQII