X-ray induction of micronuclei in human lymphocyte subpopulations differentiated by immunoperoxidase staining.
X-ray induction of micronuclei in human lymphocyte subpopulations differentiated by immunoperoxidase staining.
复制标题
通过免疫过氧化物酶染色区分的人淋巴细胞亚群中微核的 X 射线诱导。
DOI：
10.2307/3578317
复制
发表时间：
1992
影响因子：
3.4
通讯作者：
J. Cologne
中科院分区：
文献类型：
--
作者：
Sadayuki Ban;J. Cologne
在这项研究中，我们试图通过微核试验来确认人类外周血淋巴细胞亚群的辐射敏感性。从外周血中分离单个核细胞，用X射线照射。培养3d后固定细胞，用免疫过氧化物酶染色技术进行染色。用抗Leu4(CD3)、Leu2a(CD8)和Leu19(CD56)的单抗鉴定淋巴细胞亚群。通过对与特定抗体反应的双核细胞微核数量的计分，获得剂量-反应曲线，然后进行染色。CD8+(抑制性/细胞毒性)细胞的剂量效应与CD3+(PAN T)细胞非常相似。相比之下，CD56+(自然杀伤细胞)细胞的敏感性明显降低，尽管可评分的双核CD56+细胞在双核细胞总数中所占比例不到4%。
In this study we sought to confirm the radiosensitivity of human peripheral blood lymphocyte subpopulations using a micronucleus assay. Mononucleated cells isolated from peripheral blood were irradiated with X rays. After being cultured for 3 days, cells were fixed and stained using the immunoperoxidase staining technique. Lymphocyte subpopulations were characterized by means of the monoclonal antibodies Leu4 (CD3), Leu2a (CD8) and Leu19 (CD56). Dose-response curves were obtained by scoring the number of micronuclei in binucleated cells that reacted with a specific antibody and were then stained. The dose response of CD8+ (suppressor/cytotoxic) cells was quite similar to that of CD3+ (pan T) cells. In comparison, CD56+ (natural killer) cells were significantly less sensitive, although scorable binucleated CD56+ cells made up less than 4% of the total number of binucleated cells.
来自患有易患辐射诱发癌症的遗传性疾病家族的细胞株的辐射敏感性。
影响因子：
11.2
作者：
Little,JB;Nichols,WW;Troilo,P;Nagasawa,H;Strong,LC
通讯作者：
Strong,LC
低剂量照射对未刺激和 PHA 刺激的人类淋巴细胞亚群的影响。
DOI：
10.1080/09553008814550441
发表时间：
1988
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
Stewart,CC;Stevenson,AP;Habbersett,RC
通讯作者：
Habbersett,RC