Electrical stimulation of microbial PCB degradation in sediment.
Electrical stimulation of microbial PCB degradation in sediment.
复制标题
DOI：
10.1016/j.watres.2012.09.038
复制
发表时间：
2013-01-01
期刊：
影响因子：
12.8
通讯作者：
May HD
中科院分区：
文献类型：
--
作者：
Chun CL;Payne RB;Sowers KR;May HD
多氯联苯（PCB）的生物修复部分由于缺乏成本效益高的方法来刺激原位微生物降解而被排除在外。一个共同的限制是缺乏一个有效的方法提供电子供体和受体，以促进原位多氯联苯生物降解。施加电势到土壤/沉积物中可能是为多氯联苯脱氯和降解微生物提供电子供体/受体的有效手段。在这项研究中，电刺激的微生物PCB脱氯/降解进行了研究，在模拟原位条件下保持在沉积物。电压施加到开放的微观世界充满了多氯联苯影响（Aroclor 1242）淡水沉积物从超级基金网站（福克斯河，威斯康星州）。施加低电压（1.5至3.0V）对微生物转化的多氯联苯的效果进行了测定：1）加标多氯联苯，2）土著风化多氯联苯。结果表明，氧化和还原微生物转化的加标PCBs的刺激，但氧化是占主导地位的，最有效的电压越高。氯苯甲酸酯的氧化代谢产物的加标多氯联苯，但增加电压增强氯苯甲酸酯的消费，表明整体降解增强。在风化的多氯联苯的情况下，总浓度下降40-60%，暴露于电流的缩影，而没有显着减少的PCB浓度在控制反应器（0 V或消毒）观察。对风化多氯联苯的单一同系物分析显示，二氯至五氯同系物显着损失，这表明微生物活性不仅限于高氯化同系物的厌氧脱氯。降解是最明显的，只有1.5 V的应用程序，其中阳极O2不产生，表明降解的机制独立于电解O2。在这项研究中观察到的风化多氯联苯的微生物降解的低电压刺激表明，这种方法可能是一种具有成本效益的，环境可持续的策略，以修复多氯联苯在原位。
Bioremediation of polychlorinated biphenyls (PCBs) has been precluded in part by the lack of a cost-effective method to stimulate microbial degradation in situ. A common limitation is the lack of an effective method of providing electron donors and acceptors to promote in situ PCB biodegradation. Application of an electric potential to soil/sediment could be an effective means of providing electron-donors/-acceptors to PCB dechlorinating and degrading microorganisms. In this study, electrical stimulation of microbial PCB dechlorination/ degradation was examined in sediment maintained under simulated in situ conditions. Voltage was applied to open microcosms filled with PCB-impacted (Aroclor 1242) freshwater sediment from a Superfund site (Fox River, WI). The effect of applied low voltages (1.5 to 3.0V) on the microbial transformation of PCBs was determined with: 1) spiked PCBs, and 2) indigenous weathered PCBs. The results indicate that both oxidative and reductive microbial transformation of the spiked PCBs was stimulated but oxidation was dominant and most effective with higher voltage. Chlorobenzoates were produced as oxidation metabolites of the spiked PCBs, but increasing voltage enhanced chlorobenzoate consumption, indicating that overall degradation was enhanced. In the case of weathered PCBs, the total concentration decreased 40–60% in microcosms exposed to electric current while no significant decrease of PCB concentration was observed in control reactors (0 V or sterilized). Single congener analysis of the weathered PCBs showed significant loss of di- to penta-chlorinated congeners, indicating that microbial activity was not limited to anaerobic dechlorination of only higher chlorinated congeners. Degradation was most apparent with the application of only 1.5 V where anodic O2 was not generated, indicating a mechanism of degradation independent of electrolytic O2. Low voltage stimulation of the microbial degradation of weathered PCBs observed in this study suggests that this approach could be a cost-effective, environmentally sustainable strategy to remediate PCBs in situ.
登录
查看更多内容
影响因子：
4.2
作者：
Luepromchai, E;Singer, AC;Crowley, DE
通讯作者：
Crowley, DE
影响因子：
11.4
作者：
Nebe, Jennifer;Baldwin, Brett R.;Nakatsu, Cindy H.
通讯作者：
Nakatsu, Cindy H.
影响因子：
4.4
作者：
Leigh, MB;Prouzová, P;Fletcher, JS
通讯作者：
Fletcher, JS
影响因子：
3.6
作者：
HUDLICKY, M
通讯作者：
HUDLICKY, M
影响因子：
11.4
作者：
Aulenta, Federico;Catervi, Alessandro;Rossetti, Simona
通讯作者：
Rossetti, Simona