Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the α7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response.
Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the α7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response.
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DOI：
10.1155/2021/5526940
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发表时间：
2021
影响因子：
4.6
通讯作者：
Torsoni MA
中科院分区：
文献类型：
--
作者：
Martins ICA;Contieri LS;Amaral CL;Costa SO;Souza ACP;Ignacio-Souza LM;Milanski M;Torsoni AS;Torsoni MA
该研究旨在研究PUFA补充是否可以预防短期HFD对α 7 nAChR表达和脓毒症严重程度的影响。Swiss小鼠用于体内实验。对于体外实验，我们使用了来自小鼠的小胶质细胞系（BV-2）和肝癌细胞系（Hepa-1c 1c 7）。动物喂食标准食物，喂食短期HFD（60%），或补充omega-3脂肪酸（2 g/kg或4 g/kg bw）17天，然后短期HFD。用腹膜内（i. p.）脂多糖注射（LPS，5或12 mg/kg），并通过使动物进行盲肠结扎和穿孔（CLP）来诱导脓毒症。用LPS（分别为100和500 ng/mL）处理BV-2和Hepa-1c 1c 7细胞3小时。采用RT-PCR或Western印迹法评价α 7 nAChR表达、炎症标志物、DNMT 1和整体泛素化。LPS和HFD可降低α 7 nAChR的表达，增加炎症标志物的表达。Omega-3部分防止了HFD对骨髓和下丘脑中α 7 nAChR表达造成的损害，降低了炎症标志物，并降低了对脓毒症诱导死亡的敏感性。LPS诱导BV-2细胞DNMT 1蛋白含量增加，α 7 nAChR表达降低，泛素化水平升高。LPS诱导的BV-2细胞炎症反应使α 7 nAChR表达降低，DNMT 1表达增加，泛素化蛋白水平升高，提示转录前和转录后机制参与了炎症反应。
The study is aimed at investigating if PUFA supplementation could prevent the effects of a short-term HFD on α7nAChR expression and on the severity of sepsis. Swiss mice were used for the in vivo experiments. For the in vitro experiments, we used a microglia cell line (BV-2) and a hepatoma cell line (Hepa-1c1c7) derived from mice. The animals were either fed standard chow, fed a short-term HFD (60%), or given supplementation with omega-3 fatty acid (2 g/kg or 4 g/kg bw) for 17 days, followed by a short-term HFD. Endotoxemia was induced with an intraperitoneal (i.p.) lipopolysaccharide injection (LPS, 5 or 12 mg/kg), and sepsis was induced by subjecting the animals to cecal ligation and puncture (CLP). BV-2 and Hepa-1c1c7 cells were treated with LPS (100 and 500 ng/mL, respectively) for 3 hours. RT-PCR or Western blotting was used to evaluate α7nAChR expression, inflammatory markers, DNMT1, and overall ubiquitination. LPS and HFD reduced the expression of α7nAChR and increased the expression of inflammatory markers. Omega-3 partially prevented the damage caused by the HFD to the expression of α7nAChR in the bone marrow and hypothalamus, decreased the inflammatory markers, and reduced susceptibility to sepsis-induced death. Exposing the BV-2 cells to LPS increased the protein content of DNMT1 and the overall ubiquitination and reduced the expression of α7nAChR. The inflammation induced by LPS in the BV-2 cell decreased α7nAChR expression and concomitantly increased DNMT1 expression and the ubiquitinated protein levels, indicating the participation of pre- and posttranscriptional mechanisms.
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影响因子：
9.3
作者：
O'Mahoney LL;Matu J;Price OJ;Birch KM;Ajjan RA;Farrar D;Tapp R;West DJ;Deighton K;Campbell MD
通讯作者：
Campbell MD
影响因子：
9.3
作者：
Pintado C;Gavilán MP;Gavilán E;García-Cuervo L;Gutiérrez A;Vitorica J;Castaño A;Ríos RM;Ruano D
通讯作者：
Ruano D
影响因子：
5.6
作者：
De Boer, Anna A.;Monk, Jennifer M.;Robinson, Lindsay E.
通讯作者：
Robinson, Lindsay E.
影响因子：
3.1
作者：
Canastar, Andrew;Logel, Judith;Leonard, Sherry
通讯作者：
Leonard, Sherry
影响因子：
7.3
作者：
Parras Souza, Anelise Cristina;Souza, Camilla Mendes;Torsoni, Marcio Alberto
通讯作者：
Torsoni, Marcio Alberto