Rice stripe virus-derived siRNAs play different regulatory roles in rice and in the insect vector Laodelphax striatellus.
Rice stripe virus-derived siRNAs play different regulatory roles in rice and in the insect vector Laodelphax striatellus.
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水稻条纹病毒衍生的 siRNA 在水稻和昆虫载体灰飞虱中发挥不同的调节作用。
DOI：
10.1186/s12870-018-1438-7
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发表时间：
2018-10-04
影响因子：
5.3
通讯作者：
Cui F
中科院分区：
文献类型：
--
作者：
Yang M;Xu Z;Zhao W;Liu Q;Li Q;Lu L;Liu R;Zhang X;Cui F
大多数植物病毒依靠媒介昆虫传播。在病毒感染后，病毒衍生的小干扰RNA（vsiRNA）可以靶向病毒和宿主转录物。水稻条纹病毒（Rice stripe virus，RSV）是由灰飞虱（Laodelphax striatellus，Fallen）传播的一种持续繁殖的病毒，可引起水稻的严重病害。为了研究vsiRNA如何调节宿主植物和昆虫载体中的基因表达，我们分析了小RNA（sRNAs）和mRNA在RSV感染的水稻和感染的稻飞虱中的表达谱。我们在水稻中获得了88，247个vsiRNA，它们主要来自RSV RNA片段的末端区域，在稻飞虱中获得了351，655个vsiRNA，它们在RSV RNA片段上显示出相对均匀的分布。在水稻和稻飞虱中分别发现了38，112和80，698个独特的vsiRNA，而在两者中发现了14，006个独特的vsiRNA。与对照相比，感染RSV的水稻中有273个基因表达显著下调，其中192个（70.3%）是vsiRNA的潜在靶基因。基因本体论（GO）分析显示，这192个DRG富含激酶活性，碳水化合物结合和蛋白质结合相关的基因。同样，在RSV感染的稻飞虱中鉴定出265个DRG，其中126个（47.5%）是vsiRNA的潜在靶点。这些稻飞虱靶基因中富含与角质层结构组成、丝氨酸型内肽酶活性和氧化还原酶活性相关的基因。综上所述，我们的结果表明，相同病毒的感染可以在不同的宿主中产生不同的vsiRNA，从而可能调节不同的生物学过程，从而反映出不同的病毒-宿主相互作用。本文的在线版本（10.1186/s12870-018-1438-7）包含补充材料，可供授权用户使用。
Most plant viruses depend on vector insects for transmission. Upon viral infection, virus-derived small interfering RNAs (vsiRNAs) can target both viral and host transcripts. Rice stripe virus (RSV) is a persistent-propagative virus transmitted by the small brown planthopper (Laodelphax striatellus, Fallen) and can cause a severe disease on rice. To investigate how vsiRNAs regulate gene expressions in the host plant and the insect vector, we analyzed the expression profiles of small RNAs (sRNAs) and mRNAs in RSV-infected rice and RSV-infected planthopper. We obtained 88,247 vsiRNAs in rice that were predominantly derived from the terminal regions of the RSV RNA segments, and 351,655 vsiRNAs in planthopper that displayed relatively even distributions on RSV RNA segments. 38,112 and 80,698 unique vsiRNAs were found only in rice and planthopper, respectively, while 14,006 unique vsiRNAs were found in both of them. Compared to mock-inoculated rice, 273 genes were significantly down-regulated genes (DRGs) in RSV-infected rice, among which 192 (70.3%) were potential targets of vsiRNAs based on sequence complementarity. Gene ontology (GO) analysis revealed that these 192 DRGs were enriched in genes involved in kinase activity, carbohydrate binding and protein binding. Similarly, 265 DRGs were identified in RSV-infected planthoppers, among which 126 (47.5%) were potential targets of vsiRNAs. These planthopper target genes were enriched in genes that are involved in structural constituent of cuticle, serine-type endopeptidase activity, and oxidoreductase activity. Taken together, our results reveal that infection by the same virus can generate distinct vsiRNAs in different hosts to potentially regulate different biological processes, thus reflecting distinct virus-host interactions. The online version of this article (10.1186/s12870-018-1438-7) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.7
作者：
Donaire, Livia;Wang, Yu;Llave, Cesar
通讯作者：
Llave, Cesar
影响因子：
11.4
作者：
Bouche, Nicolas;Lauressergues, Dominique;Vaucheret, Herve
通讯作者：
Vaucheret, Herve
影响因子：
11.6
作者：
Diaz-Pendon, Juan A.;Li, Feng;Ding, Shou-Wei
通讯作者：
Ding, Shou-Wei
影响因子：
9.8
作者：
John B;Enright AJ;Aravin A;Tuschl T;Sander C;Marks DS
通讯作者：
Marks DS