Comparative physiological and transcriptomic analyses of photosynthesis in Sphagneticola calendulacea (L.) Pruski and Sphagneticola trilobata (L.) Pruski.
Comparative physiological and transcriptomic analyses of photosynthesis in Sphagneticola calendulacea (L.) Pruski and Sphagneticola trilobata (L.) Pruski.
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金盏泥炭藓 (Sphagneticola calendulacea (L.) Pruski) 和三叶泥炭藓 (Sphagneticola trilobata (L.) Pruski) 光合作用的比较生理和转录组分析
DOI：
10.1038/s41598-020-74289-1
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发表时间：
2020-10-20
影响因子：
4.6
通讯作者：
Peng CL
中科院分区：
文献类型：
--
作者：
Cai ML;Zhang QL;Zhang JJ;Ding WQ;Huang HY;Peng CL
三裂球孢霉Sphagneticola trilobata（L.）草螟是我国南方生长迅速的恶性杂草之一。它严重影响了当地的生物多样性和原生植物栖息地。光合作用是植物生长发育的物质基础。然而，目前关于S.三裂叶。在本研究中，S. trilobata具有相对较大的叶面积和生物量。叶片单位面积的气体交换参数，包括净光合能力（Pn）、胞间CO2（Ci）、气孔导度（Gs）、蒸腾速率（Tr）、水分利用效率（WUE）、光合色素和Rubisco蛋白含量均高于乡土植物Sphagneticola acuulacea（L.）普鲁斯基在此基础上，利用Illumina HiSeq平台分析了两种球孢霉光合作用途径的差异。序列测定结果表明，S. trilobata和S.单基因组分别为159，366个和177，069个。功能注释揭示了119，350和150，846个非冗余蛋白质数据库注释（Nr），96，637和115，711个Swiss-Prot注释，49，159和60，116个京都基因和基因组百科全书注释（KEGG），以及83，712和97，957个S. trilobata和S.分别为紫云英科。此外，我们的分析表明，参与光合作用途径的关键蛋白酶基因，特别是CP 43，CP 47，PsbA和PetC，在S.与本地物种相比，生理和转录组学分析表明，光合基因的高表达保证了叶片的高光合能力，这是S.三裂叶。
Sphagneticola trilobata (L.) Pruski is one of the fast-growing malignant weeds in South China. It has severely influenced local biodiversity and native plant habitat. Photosynthesis is the material basis of plant growth and development. However, there are few reports on the photosynthetic transcriptome of S. trilobata. In this study, S. trilobata had a relatively large leaf area and biomass. The gas exchange parameters per unit area of leaves, including net photosynthetic capacity (Pn), intercellular CO2 (Ci), stomatal conductance (Gs), transpiration rate (Tr), water use efficiency (WUE), photosynthetic pigment and Rubisco protein content were higher than those of the native plant Sphagneticola calendulacea (L.) Pruski. On this basis, the differences in photosynthesis pathways between the two Sphagneticola species were analyzed by using the Illumina HiSeq platform. The sequencing results for S. trilobata and S. calendulacea revealed 159,366 and 177,069 unigenes, respectively. Functional annotation revealed 119,350 and 150,846 non-redundant protein database annotations (Nr), 96,637 and 115,711 Swiss-Prot annotations, 49,159 and 60,116 Kyoto Encyclopedia of Genes and Genomes annotations (KEGG), and 83,712 and 97,957 Gene Ontology annotations (GO) in S. trilobata and S. calendulacea, respectively. Additionally, our analysis showed that the expression of key protease genes involved in the photosynthesis pathway, particularly CP43, CP47, PsbA and PetC, had high expression levels in leaves of S. trilobata in comparison to native species. Physiological and transcriptomic analyses suggest the high expression of photosynthetic genes ensures the high photosynthetic capacity of leaves, which is one of the inherent advantages underlying the successful invasion by S. trilobata.
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DOI：
10.3390/antiox2040340
发表时间：
2013-11-26
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Racchi ML
通讯作者：
Racchi ML
影响因子：
1.4
作者：
Li, G. L.;Wu, H. X.;Zhang, S. Y.
通讯作者：
Zhang, S. Y.
影响因子：
1.5
作者：
Li, Liqin;Xu, Li;Lu, Liming
通讯作者：
Lu, Liming
影响因子：
0.9
作者：
Li, Ting;Huang, Li-Xin;Wang, Zhang-Ming
通讯作者：
Wang, Zhang-Ming
缺钾甘蓝型油菜叶片和长角果光合及结构差异
影响因子：
5.3
作者：
Lu Z;Pan Y;Hu W;Cong R;Ren T;Guo S;Lu J
通讯作者：
Lu J