Positive selection of digestive proteases in Daphnia: A mechanism for local adaptation to cyanobacterial protease inhibitors
Positive selection of digestive proteases in Daphnia: A mechanism for local adaptation to cyanobacterial protease inhibitors
复制标题
水蚤消化蛋白酶的正选择：局部适应蓝藻蛋白酶抑制剂的机制
DOI：
10.1111/mec.15375
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发表时间：
2020
影响因子：
4.9
通讯作者：
von Elert
中科院分区：
文献类型：
--
作者：
Schwarzenberger;Hasselmann;von Elert
由于全球变暖和富营养化的综合影响，淡水生态系统中有害蓝藻水华的频率增加。与此相一致的是，水生关键食草动物Daphniato蓝藻的本地适应性受到了极大的关注。除了微囊藻毒素，在这种水华中最常见的蓝藻次级代谢产物是蛋白酶抑制剂（PI）。最近，已经表明蛋白酶基因在对PI的耐受性不同的四个大果蝇种群之间显示出拷贝数变异。从这项研究中，我们选择了两个不同的D. magna种群，它们过去曾与蓝细菌共存或未与蓝细菌共存。通过计算FST值，我们发现两个群体在蛋白酶位点上的遗传距离比在中性位点上的遗传距离更远。群体遗传测试表明，正选择最有可能作用于消化蛋白酶CT448和CT802的基因位点。我们的结论是，选择消化蛋白酶和随后的拷贝数减少的进化变化的分子基础，导致当地适应PI。
Due to the combined effects of global warming and eutrophication, the frequency of deleterious cyanobacterial blooms in freshwater ecosystems has increased. In line with this, local adaptation of the aquatic keystone herbivoreDaphniato cyanobacteria has received major attention. Besides microcystins, the most frequent cyanobacterial secondary metabolites in such blooms are protease inhibitors (PIs). Recently, it has been shown that a protease gene showed copy number variation between fourD. magnapopulations that differed in tolerance to PIs. From that study, we chose two distinct populations ofD. magnawhich had or had not coexisted with cyanobacteria in the past. By calculatingFSTvalues, we found that the two populations were genetically more distant in the protease loci than in neutral loci. Population genetic tests applied to the tolerant population revealed that positive selection was most probably acting on the gene loci of the digestive protease CT448 and CT802. We conclude that the selection of digestive proteases and subsequent reduction in copy number is the molecular basis of evolutionary changes leading to local adaptation to PIs.
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解解微囊藻蛋白耐受性的遗传基础。
影响因子：
4.4
作者：
Schwarzenberger A;Sadler T;Motameny S;Ben-Khalifa K;Frommolt P;Altmüller J;Konrad K;von Elert E
通讯作者：
von Elert E
影响因子：
2.7
作者：
P. Hebert;T. Finston
通讯作者：
T. Finston
影响因子：
3.3
作者：
Yunxin Fu
通讯作者：
Yunxin Fu
蓝藻营养限制对大型溞蛋白酶抑制剂产生和适应性的影响
影响因子：
2.8
作者：
A. Schwarzenberger;Thomas Sadler;E. von Elert
通讯作者：
E. von Elert
大型湖泊水蚤种群的遗传结构
影响因子：
3.3
作者：
M. Mort;H. Wolf
通讯作者：
H. Wolf