Cyclic AMP stimulates protein synthesis in L6 myoblasts and its effects are additive to those of insulin, vasopressin and 12-0-tetradecanoylphorbol-13-acetate. Possible involvement of mitogen activated protein kinase.
Cyclic AMP stimulates protein synthesis in L6 myoblasts and its effects are additive to those of insulin, vasopressin and 12-0-tetradecanoylphorbol-13-acetate. Possible involvement of mitogen activated protein kinase.
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环 AMP 刺激 L6 成肌细胞中的蛋白质合成，其作用与胰岛素、加压素和 12-0-十四烷酰佛波醇-13-乙酸酯的作用相加。
DOI：
10.1016/0167-4889(95)00194-8
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
R. Palmer
中科院分区：
文献类型：
--
作者：
M. Thompson;S. Mackie;A. Thom;D. Hazlerigg;K. Morrison;R. Palmer
在L 6成肌细胞中研究了环AMP作为第二信使在刺激蛋白质合成中的作用以及有丝分裂原活化蛋白（MAP）激酶在这种反应中的潜在参与。二丁酰-cAMP（dbt-cAMP）在90分钟和6小时的蛋白质合成增加浓度依赖性的方式。在90分钟的反应可能是介导的翻译增加，因为它们没有被放线菌素D阻断，在6小时的影响伴随着RNA含量的增加，这意味着转录成分。100 nM 12-0-tetradecanoylphorbol-13-acetate（TPA）、1 nM胰岛素（孵育90 min）和100 nM加压素（孵育6 h）也可增加蛋白质合成，这些反应与500 μM dbt-cAMP的反应相加。对毛喉素的反应类似于dbt-cAMP，而1，9-二脱氧毛喉素没有影响。用MAP激酶抗体免疫印迹的细胞提取物显示对应于约100 μ g/ml的条带。42、44、54和83 kDa。500 μM dbt-cAMP引起42和44 kDa条带的活性增加，当用"凝胶“法测定时，用毛喉素也观察到类似的反应。TPA和加压素也刺激这两种亚型的活性，但当与500 μM dbt-cAMP联合使用时，没有显著的相加或抑制作用。相比之下，尽管单独使用1 nM胰岛素没有效果，但在dbt-cAMP存在下观察到MAP激酶活化方面的协同反应。数据表明，cAMP刺激L 6细胞中的蛋白质合成，并表明MAP激酶在此事件中的作用。
The role of cyclic AMP as a second messenger in the stimulation of protein synthesis and the potential involvement of mitogen activated protein (MAP) kinase in this response was studied in L6 myoblasts. Dibutyryl-cAMP (dbt-cAMP) increased protein synthesis at 90 min and 6 h in a concentration-dependent manner. The responses at 90 min were probably mediated by increased translation as they were not blocked by actinotnycin D; effects at 6 h were accompanied by increases in RNA content implying a transcriptional component. 100 nM 12-0-tetradecanoylphorbol-13-acetate (TPA), 1 nM Insulin (90 min incubations) and 100 nM vasopressin (6 h incubation) also increased protein synthesis and these responses were additive with those of 500 μM dbt-cAMP. Responses to forskolin were similar to dbt-cAMP whilst 1,9-dideoxyforskolin had no effect. Cell extracts immunoblotted with MAP kinase antibody showed bands corresponding to approx. 42, 44, 54 and 83 kDa. 500 μM dbt-cAMP elicited an increase in activity of both the 42 and 44 kDa bands when assayed by the ‘in gel’ method and a similar response was also observed with forskolin. TPA and vasopressin also stimulated the activity of these two isoforms, but had no significant additive or inhibitory effects when added in combination with 500 μM dbt-cAMP. In contrast, although 1 nM insulin alone; had no effect, a synergistic response in terms of MAP kinase activation was observed in the presence of dbt-cAMP. The data demonstrate that cAMP stimulates protein synthesis in L6 cells and suggest a role for MAP kinase in this event.
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影响因子：
56.9
作者：
COOK, SJ;MCCORMICK, F
通讯作者：
MCCORMICK, F
DOI：
10.1016/s0021-9258(18)82362-6
发表时间：
1993-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Davis
通讯作者：
R. Davis
GLUT4 磷酸化和二丁酰 cAMP 对葡萄糖转运的抑制。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Piper,RC;James,DE;Slot,JW;Puri,C;LawrenceJr,JC
通讯作者：
LawrenceJr,JC
影响因子：
56.9
作者：
WU, J;DENT, P;STURGILL, TW
通讯作者：
STURGILL, TW
影响因子：
8
作者：
Kondo, T;Minamino, N;Tanaka, N
通讯作者：
Tanaka, N