Diverse and divergent protein post-translational modifications in two growth stages of a natural microbial community.
Diverse and divergent protein post-translational modifications in two growth stages of a natural microbial community.
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DOI：
10.1038/ncomms5405
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发表时间：
2014-07-25
影响因子：
16.6
通讯作者：
Pan, Chongle
中科院分区：
文献类型：
--
作者：
Li, Zhou;Wang, Yingfeng;Yao, Qiuming;Justice, Nicholas B.;Ahn, Tae-Hyuk;Xu, Dong;Hettich, Robert L.;Banfield, Jillian F.;Pan, Chongle
微生物群落中蛋白质翻译后修饰（PTM）的详细表征仍然是一个重大挑战。在这里，我们直接识别和量化酸性矿山排水场天然微生物群落中的各种 PTM（羟基化、甲基化、瓜氨酸化、乙酰化、磷酸化、甲硫基化、S-亚硝基化和硝化）。在主要的钩端螺旋菌 II 类细菌中，大约 29% 的已鉴定蛋白质被修饰，并且 43% 的修饰蛋白质携带多种 PTM 类型。大多数 PTM 事件（S-亚硝基化除外）的占有率较低。值得注意的是，PTM 事件是在参与抗病毒防御的 Cas 蛋白上检测到的，这是之前未考虑到的 Cas 生物化学的一个方面。此外，II 组钩端螺旋菌的 Cas PTM 谱在早期生物膜与成熟生物膜中存在差异。两种密切相关但生态上不同的钩端螺旋菌 II 类细菌的直系同源物的 PTM 模式有所不同。我们的结果强调了蛋白质 PTM 的普遍性和动态，对生态适应和微生物进化具有潜在意义。 表征微生物群落中蛋白质的翻译后修饰具有挑战性。在这里，作者鉴定并量化了天然微生物生物膜的两个生长阶段中此类修饰的大量和多样性。
Detailed characterization of post-translational modifications (PTMs) of proteins in microbial communities remains a significant challenge. Here we directly identify and quantify a broad range of PTMs (hydroxylation, methylation, citrullination, acetylation, phosphorylation, methylthiolation, S-nitrosylation and nitration) in a natural microbial community from an acid mine drainage site. Approximately 29% of the identified proteins of the dominant Leptospirillum group II bacteria are modified, and 43% of modified proteins carry multiple PTM types. Most PTM events, except S-nitrosylations, have low fractional occupancy. Notably, PTM events are detected on Cas proteins involved in antiviral defense, an aspect of Cas biochemistry not considered previously. Further, Cas PTM profiles from Leptospirillum group II differ in early versus mature biofilms. PTM patterns are divergent on orthologues of two closely related, but ecologically differentiated, Leptospirillum group II bacteria. Our results highlight the prevalence and dynamics of PTMs of proteins, with potential significance for ecological adaptation and microbial evolution. Characterizing post-translational modifications of proteins in microbial communities is challenging. Here, the authors identify and quantify a great number and diversity of such modifications in two growth stages of a natural microbial biofilm.
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影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
48
作者：
Mertins, Philipp;Qiao, Jana W.;Patel, Jinal;Udeshi, Namrata D.;Clauser, Karl R.;Mani, D. R.;Burgess, Michael W.;Gillette, Michael A.;Jaffe, Jacob D.;Carr, Steven A.
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Banfield, Jillian F.
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作者：
Andersson, Anders F.;Banfield, Jillian F.
通讯作者：
Banfield, Jillian F.
影响因子：
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作者：
Benndorf, Dirk;Balcke, Gerd U.;von Bergen, Martin
通讯作者：
von Bergen, Martin