Selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene.
Selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene.
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通过与口腔卫生不良相关的酶活性选择性调节细菌附着于口腔上皮细胞。
DOI：
10.1111/j.1600-0765.1990.tb01040.x
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发表时间：
1990
影响因子：
3.5
通讯作者：
Gibbons,RJ
中科院分区：
文献类型：
--
作者：
Childs3rd,WC;Gibbons,RJ
本研究探讨了这样一种假设，即口腔卫生差和/或牙龈炎症的个体的牙龈沟环境中某些酶的水平升高可能会改变上皮细胞的表面，从而调节附着和定植的细菌类型。以神经氨酸酶和特定蛋白酶处理的口腔上皮细胞为模型进行研究。研究的细菌包括与牙龈健康相关的血链球菌和轻微链球菌，与牙龈炎相关的菌斑中增加的放线菌，以及与破坏性牙周病相关的牙龈拟杆菌、中间杆菌和放线菌。用所研究的酶处理上皮细胞对细菌的接受性产生选择性影响。上皮细胞的神经氨酸酶处理大大降低了所有菌株的附着。sanguisandS。mitisstudied。与此相反，粘酵母菌、A. naeslundiiandA。附着的以色列细胞显著增加。神经氨酸酶处理似乎也增强了b的附着。intermediusandB。gingivalis。用胰蛋白酶、凝乳胰蛋白酶或木瓜蛋白酶处理颊细胞也选择性地影响细菌附着。这种蛋白酶处理大大减少了链球菌的数量。黏度是附着的细胞数，而b。gingivalisandB。中间值显著增加。来源于人PMNs的溶酶体酶制剂对上皮细胞的处理也产生了类似的选择性作用。酶治疗所观察到的细菌粘附的变化与牙龈缝隙菌群组成的变化是一致的，这种变化发生在口腔卫生终止和牙龈炎发生时。
The present investigation explored the hypothesis that elevated levels of certain enzymes in the gingival crevicular environment of individuals with poor oral hygiene and/or gingival inflammation may modify the surfaces of epithelial cells and thereby modulate the types of bacteria which attach and colonize. Buccal epithelial cells treated with neuraminidase and certain proteases were used as a model for study. Bacteria studied includedStreptococcus sanguisandStreptococcus mitiswhich have been associated with gingival health,Actinomycesspecies which are increased in plaque associated with developing gingivitis, andBacteroides gingivalis, Bacteraides intermedius, andActinobacillus actinomy‐cetemcomitanswhich are associated with destructive periodontal diseases. Treatment of epithelial cells with the enzymes studied produced selective effects on their receptivity for bacteria. Neuraminidase treatment of epithelial cells greatly reduced the attachment of all strains ofS. sanguisandS. mitisstudied. In contrast, the number ofActinomyces viscosus, A. naeslundiiandA. israeliicells which attached was significantly increased. Neuraminidase treatment also appeared to enhance attachment ofB. intermediusandB. gingivalis. Treatment of buccal cells with trypsin, chymotrypsin or papain also selectively affected bacterial attachment. Such protease treatment greatly reduced the numbers of streptococci andA. viscosuscells which attached, while the numbers ofB. gingivalisandB. intermediuswere significantly increased. Treatment of epithelial cells with preparations of lysosomal enzymes derived from human PMNs produced similar selective effects. The changes in bacterial adhesion observed by the enzyme treatments studied are consistent with the shifts in the composition of the gingival crevice flora which occur when oral hygiene is terminated and gingivitis develops.
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影响铜绿假单胞菌与哺乳动物颊上皮细胞粘附的因素。
DOI：
10.1093/clinids/5.supplement_5.s846
发表时间：
1983
期刊：
Reviews of infectious diseases
影响因子：
--
作者：
Woods,DE;Straus,DC;JohansonJr,WG;Bass,JA
通讯作者：
Bass,JA
牙龈拟杆菌培养上清血凝素的纯化及性质
影响因子：
3.1
作者：
K. Okuda;A. Yamamoto;Y. Naito;I. Takazoe;J. Slots;R. Genco
通讯作者：
R. Genco
影响因子：
3.1
作者：
ELLEN, RP;FILLERY, ED;GROVE, DA
通讯作者：
GROVE, DA
人裂隙液中的神经氨酸酶活性。
影响因子：
3.5
作者：
M. Kitawaki;K. Iijima;T. Nakashizuka;T. Hayakawa
通讯作者：
T. Hayakawa
唾液-细菌相互作用的特异性：II. 血链球菌凝集素对 NeuAcα2,3Ga1β1,3Ga1NAc 序列具有特异性的证据
DOI：
--
发表时间：
1982
期刊：
影响因子：
--
作者：
P. Murray;M. Levine;L. Tabak;M. Reddy
通讯作者：
M. Reddy