Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts.
Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts.
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牙源性角化囊肿囊液中细胞源性微粒水平增加
DOI：
10.3892/ijo.2018.4361
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发表时间：
2018-06
影响因子：
5.2
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Man QW;Zhong WQ;Ren JG;Liu JY;Zheng YY;Li RF;Nyimi BF;Chen G;Zhao YF;Liu B
本研究的目的是检测牙源性角化囊肿（OKC）囊液中细胞源性微粒（MP）的水平和基本特征。为此，从OKC的囊液（CFMPs）的MPs纯化通过经典的差速离心法，并通过透射电子显微镜和荧光显微镜进行了表征。流式细胞术分析用于确定CFMP的大小、浓度和细胞来源。免疫组化检测OKC中核因子-κB配体受体激活剂的表达水平，并采用斯皮尔曼秩相关检验分析其与CFMPs浓度的相关性。此外，进行逆转录-定量聚合酶链反应（RT-qPCR）和抗酒石酸酸性磷酸酶（TRAP）染色，以检查小鼠骨髓源性巨噬细胞（BMPs）对CFMP的破骨细胞生成反应。结果显示，与含牙囊肿（DCs）和根尖囊肿（RCs）相比，OKC中总CFMPs水平显著升高。此外，体外实验进一步揭示，源自患者OKC的CFMP可被BcR摄取，导致活化T细胞核因子1（NFATc 1）和TRAP的mRNA表达水平显著增加。此外，TRAP阳性的多核破骨细胞在巨噬细胞集落刺激因子（M-CSF）和CFMPs与Bcl 3的存在下成功地培养。总体而言，我们的研究结果表明，OKC患者的CFMPs水平高于DC和RC患者，这可能与OKC的骨吸收有关。
The aim of this study was to examine the level and basic characteristics of cell-derived microparticles (MPs) in the cyst fluids of odontogenic keratocysts (OKCs). For this purpose, MPs from the cyst fluids (CFMPs) of OKCs were purified by a classic differential centrifugation method and characterized by a transmission electron microscope and fluorescence microscope. Flow cytometric analysis was used to determine the size, concentration and cellular origins of the CFMPs. Moreover, the expression level of receptor activator for nuclear factor-κB ligand in the OKCs was evaluated by immunohistochemical staining and then analyzed for its correlation with the concentration of CFMPs by Spearman's rank correlation test. In addition, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and tartaric-resistant acid phosphatase (TRAP) staining were performed to examine the osteoclastogenesis of mouse bone marrow-derived macrophages (BMMs) in response to CFMPs. The results revealed that the levels of total CFMPs were significantly elevated in OKCs compared with dentigerous cysts (DCs) and radicular cysts (RCs). In addition, in vitro experiments further revealed that CFMPs derived from the OKCs of patients could be taken up by BMMs, leading to a significant increase in the mRNA expression levels of nuclear factor of activated T-cells 1 (NFATc1) and TRAP. Moreover, TRAP-positive multinucleated osteoclasts were successfully cultured in the presence of macrophage colony-stimulating factor (M-CSF) and CFMPs with BMMs. On the whole, our findings indicate that patients with OKCs have higher levels of CFMPs compared with patients with DCs and RCs, which may be associated with the bone resorption of OKCs.
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DOI：
10.1097/igc.0b013e318241d9b9
发表时间：
2012-05-01
影响因子：
4.8
作者：
Press, Joshua Z.;Reyes, Morayma;Swisher, Elizabeth M.
通讯作者：
Swisher, Elizabeth M.
影响因子：
20.3
作者：
Nieuwland, R;Berckmans, RJ;Sturk, A
通讯作者：
Sturk, A
影响因子：
37.8
作者：
Mallat, Z;Hugel, B;Tedgui, A
通讯作者：
Tedgui, A
DOI：
10.1016/j.tripleo.2007.06.009
发表时间：
2008-03-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
da Silva, Tarcilia Aparecida;Batista, Aline Carvalho;Cunha, Fernando Queiroz
通讯作者：
Cunha, Fernando Queiroz
影响因子：
50.3
作者：
Becker A;Thakur BK;Weiss JM;Kim HS;Peinado H;Lyden D
通讯作者：
Lyden D