Sequence and diversity of bovine T-cell receptor β-chain genes
Sequence and diversity of bovine T-cell receptor β-chain genes
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牛T细胞受体β链基因的序列和多样性
作者：
A. Tanaka;N. Ishiguro;M. Shinagawa
从构建的牛外周血淋巴细胞cDNA文库（2 × 106噬斑）中分离出38个T细胞受体（Tcr）β链cDNA克隆，测定其核苷酸序列。在38个cDNA克隆中，有22个克隆被重排并含有功能可变区（V）基因片段。这些克隆初步分为9个Tcrb-V基因家族，对应于人Tcrb-V家族。其中，从22个克隆中的9个克隆中分离到Tcrb-V 12基因片段，表明该Tcrb-V家族在牛外周血淋巴细胞中表达。发现了两个不同的恒定（C）区，两个C区均由178个氨基酸残基组成。牛Tcrb-C区的氨基酸序列与人、小鼠和兔的相似性分别为80%-82%、78%和78%。为了评估Tcrb-V相关的限制性片段长度多态性（RFLP），使用来自四个牛品种，荷斯坦，安格斯，赫里福德，和日本黑牛的肝脏DNA进行Southern印迹分析。但Tcrb-V基因座的基因组DNA在4个品种间差异不显著。
The nucleotide sequences of 38 T-cell receptor (Tcr) β-chain cDNA clones which were isolated from a cDNA library (2 × 106 plaques) constructed from bovine peripheral blood lymphocytes were determined. Of 38 cDNA clones, 22 were rearranged and contained the functional variable (V) gene segments. These clones were tentatively divided into nine Tcrb-V gene families which correspond to the human Tcrb-V family. Among them, a Tcrb-V12 gene segment was isolated from 9 out of 22 clones, suggesting that this Tcrb-V family was expressed in the bovine peripheral blood lymphocytes. Two different constant (C) geen segments were found, and both C regions were composed of 178 amino residues. The amino acid sequences of bovine Tcrb-C regions are approximately 80%–82%, 78%, and 78% similar to those from human, mouse, and rabbit, respectively. To estimate Tcrb-V-associated restriction fragment length polymorphisms (RFLPs), Southern blot analysis was performed using liver DNAs from four bovine breeds, Holstein, Angus, Hereford, and Japanese Black. However, no significant difference was observed among genomic DNAs of Tcrb-V loci from these four breeds.
T 细胞受体基因重排作为 T 细胞肿瘤谱系和克隆性的标记。
DOI：
10.1073/pnas.82.10.3460
发表时间：
1985
影响因子：
11.1
作者：
Flug,F;Pelicci,PG;Bonetti,F;Knowles2nd,DM;Dalla-Favera,R
通讯作者：
Dalla-Favera,R
大鼠中的 T 细胞受体 β 链基因。
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Williams,CB;Gutman,GA
通讯作者：
Gutman,GA