Heterochromatin protein 1 beta regulates neural and neural crest development by repressing pluripotency-associated gene pou5f3.2/oct25 in Xenopus.
Heterochromatin protein 1 beta regulates neural and neural crest development by repressing pluripotency-associated gene pou5f3.2/oct25 in Xenopus.
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DOI：
10.1002/dvdy.319
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Chang C
中科院分区：
文献类型：
--
作者：
Tien CL;Mohammadparast S;Chang C
异染色质蛋白1(HP1)与致密染色质构象相关并在其中发挥作用，但HP1在脊椎动物胚胎发育中的作用尚不完全清楚。在这里，我们探讨了HP1在非洲爪蛙早期神经发育中的活性。我们发现，在早期胚胎的神经和神经脊衍生物中，Hp1、Hp1α，β和γ的三种异构体以相似的模式表达。尽管如此，在推测的神经组织中，hp1β和hp1γ的敲除会导致头部缺陷，而不是hp1α的敲除。在形态胚胎中，晚期泛神经标记基因和神经脊指定子基因减少，但早期神经和神经板边界基因受到的影响较小。进一步研究发现，在Hp1β变异体中，神经元分化受损，多能相关基因pou5f3.2/oct25被扩增。Pou5f3.2/oct25的异位表达模拟了hp1β基因敲除对标志物表达的影响，而同时敲除hp1β和pou5f3.2/oct25可以部分挽救这些基因的表达。综上所述，数据表明，Hp1β在非洲爪哇神经和神经脊发育过程中调节从前体细胞到更分化的细胞类型的转变，并且至少部分是通过抑制与多能性相关的转录调节因子pou5f3.2/oct25来实现的。
Heterochromatin protein 1 (HP1) is associated with and plays a role in compact chromatin conformation, but the function of HP1 in vertebrate embryogenesis is not understood completely. Here, we explore the activity of HP1 in early neural development in the frog Xenopus laevis. We show that the three isoforms of HP1, HP1α, β, and γ, are expressed in similar patterns in the neural and neural crest derivatives in early embryos. Despite this, knockdown of HP1β and HP1γ, but not HP1α, in presumptive neural tissues leads to head defects. Late pan-neural markers and neural crest specifier genes are reduced, but early neural and neural plate border genes are less affected in the morphant embryos. Further investigation reveals that neuronal differentiation is impaired and a pluripotency-associated gene, pou5f3.2/oct25, is expanded in HP1β morphants. Ectopic expression of pou5f3.2/oct25 mimics the effect of HP1β knockdown on marker expression, whereas simultaneous knockdown of HP1β and pou5f3.2/oct25 partially rescues expression of these genes. Taken together, the data suggest that HP1β regulates transition from precursor to more differentiated cell types during neural and neural crest development in Xenopus, and it does so at least partially via repression of the pluripotency-associated transcription regulator pou5f3.2/oct25.
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