Integrated anatomical and physiological mapping of striatal afferent projections.
Integrated anatomical and physiological mapping of striatal afferent projections.
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DOI：
10.1111/ejn.13829
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Fuccillo MV
中科院分区：
文献类型：
--
作者：
Choi K;Holly EN;Davatolhagh MF;Beier KT;Fuccillo MV
背内侧纹状体是奖赏敏感运动输出的关键部位，接受来自皮层、丘脑和中脑的广泛传入输入。这些投射由纹状体微电路整合，纹状体微电路包含棘状投射神经元和局部回路中间神经元。为了探索这些投射的靶细胞特异性，我们利用细胞型特异性狂犬病毒追踪和光遗传介导的小鼠投射神经元募集，比较了d1 -多巴胺受体阳性的棘神经元、parvalbumin阳性的快速尖峰中间神经元和生长抑素阳性的低阈值尖峰中间神经元的输入。虽然逆行标记的投射神经元的相对比例在不同的靶细胞类型之间是相似的，但在快速尖峰的中间神经元上，输入的收敛性更高。狂犬病毒经常被用于评估细胞特异性解剖连通性，但目前尚不清楚这与突触连通性和疗效之间的关系。为了验证这一点，我们使用新的定量光遗传学测量方法，将追踪数据与前扣带皮层和束旁丘脑投射的靶向细胞特异性突触效能测量进行了比较。我们发现靶特异性趋同模式根据投射神经元起源区域和突触后细胞类型被广泛修改。此外，我们观察到细胞类型特异性解剖连接和兴奋性突触强度测量之间的显著差异，特别是对于低阈值尖峰中间神经元。综上所述，这表明纹状体传入输入有一个基本统一的连接图，在此基础上突触前-突触后相互作用施加了实质性的生理连接多样性。
The dorsomedial striatum, a key site of reward-sensitive motor output, receives extensive afferent input from cortex, thalamus and midbrain. These projections are integrated by striatal microcircuits containing both spiny projection neurons and local circuit interneurons. To explore target-cell specificity of these projections, we compared inputs onto D1-dopamine receptor positive spiny neurons, parvalbumin-positive fast-spiking interneurons and somatostatin-positive low-threshold-spiking interneurons, using cell-type specific rabies virus tracing and optogenetic-mediated projection neuron recruitment in mice. While the relative proportion of retrogradely labeled projection neurons was similar between target cell types, the convergence of inputs was systematically higher for projections onto fast-spiking interneurons. Rabies virus is frequently used to assess cell-specific anatomical connectivity but it is unclear how this correlates to synaptic connectivity and efficacy. To test this, we compared tracing data with target cell-specific measures of synaptic efficacy for anterior cingulate cortex and parafascicular thalamic projections using novel quantitative optogenetic measures. We found that target-specific patterns of convergence were extensively modified according to region of projection neuron origin and postsynaptic cell type. Furthermore, we observed significant divergence between cell type-specific anatomical connectivity and measures of excitatory synaptic strength, particularly for low-threshold-spiking interneurons. Taken together, this suggests a basic uniform connectivity map for striatal afferent inputs upon which presynaptic-postsynaptic interactions impose substantial diversity of physiological connectivity.
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影响因子：
16.2
作者：
Straub C;Saulnier JL;Bègue A;Feng DD;Huang KW;Sabatini BL
通讯作者：
Sabatini BL
影响因子：
3.4
作者：
Lin, John Y.;Lin, Michael Z.;Tsien, Roger Y.
通讯作者：
Tsien, Roger Y.
影响因子：
25
作者：
Tai, Lung-Hao;Lee, A. Moses;Benavidez, Nora;Bonci, Antonello;Wilbrecht, Linda
通讯作者：
Wilbrecht, Linda
影响因子：
16.2
作者：
Fuccillo MV;Földy C;Gökce Ö;Rothwell PE;Sun GL;Malenka RC;Südhof TC
通讯作者：
Südhof TC
影响因子：
64.8
作者：
通讯作者：
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