Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells.
Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells.
复制标题
DOI：
10.1083/jcb.200902083
复制
发表时间：
2009-09-07
期刊：
影响因子：
--
通讯作者：
Griffis ER
中科院分区：
文献类型：
--
作者：
Vale RD;Spudich JA;Griffis ER
多个有丝分裂马达协调它们的信号，以确保肌动球蛋白收缩环在胞质分裂期间在正确的位置形成。有丝分裂后期纺锤体的信号指明了胞质分裂期间肌动球蛋白收缩环的位置，但详细的机制仍未得到解决。在这里，我们已经成像动态的绿色荧光蛋白标记的肌球蛋白丝，微管，和驱动蛋白-6（携带Rho鸟苷三磷酸酶的激活剂）在细胞皮质使用全内反射荧光显微镜在扁平的果蝇S2细胞。在分裂后期开始时，驱动蛋白-6重新定位于微管加向皮质生长的末端，但随着时间的推移，它的定位得到改善，使其集中在稳定微管的子集上，并沿着赤道处的弥散皮质带。驱动蛋白-6定位的模式非常类似于新的肌球蛋白丝通过从头组装出现在皮层的地方。当肌球蛋白在赤道处聚集时，肌球蛋白丝从细胞的两极消失，这一过程也需要驱动蛋白-6以及可能来自伸长纺锤体的其他信号。这些结果表明模型驱动蛋白-6可能如何定义皮质肌球蛋白在胞质分裂的位置。
Multiple mitotic motors coordinate their signals to ensure that the actomyosin contractile ring forms in the right place during cytokinesis. Signals from the mitotic spindle during anaphase specify the location of the actomyosin contractile ring during cytokinesis, but the detailed mechanism remains unresolved. Here, we have imaged the dynamics of green fluorescent protein–tagged myosin filaments, microtubules, and Kinesin-6 (which carries activators of Rho guanosine triphosphatase) at the cell cortex using total internal reflection fluorescence microscopy in flattened Drosophila S2 cells. At anaphase onset, Kinesin-6 relocalizes to microtubule plus ends that grow toward the cortex, but refines its localization over time so that it concentrates on a subset of stable microtubules and along a diffuse cortical band at the equator. The pattern of Kinesin-6 localization closely resembles where new myosin filaments appear at the cortex by de novo assembly. While accumulating at the equator, myosin filaments disappear from the poles of the cell, a process that also requires Kinesin-6 as well as possibly other signals that emanate from the elongating spindle. These results suggest models for how Kinesin-6 might define the position of cortical myosin during cytokinesis.
登录
查看更多内容
影响因子：
11.8
作者：
Dechant, R;Glotzer, M
通讯作者：
Glotzer, M
影响因子：
64.8
作者：
Fuller, Brian G.;Lampson, Michael A.;Kapoor, Tarun M.
通讯作者：
Kapoor, Tarun M.
影响因子：
9.2
作者：
Canman, JC;Hoffman, DB;Salmon, ED
通讯作者：
Salmon, ED
影响因子：
3.3
作者：
Goshima, G;Vale, RD
通讯作者：
Vale, RD
影响因子：
64.8
作者：
Canman, JC;Cameron, LA;Salmon, ED
通讯作者：
Salmon, ED